Improving the EMA Binding Test by Using Commercially Available Fluorescent Beads

• Published in: Frontiers in physiology Vol. 11; p. 569289
• Main Authors: Glenthøj, Andreas, Sharfo, Alaa, Brieghel, Christian, Nardo-Marino, Amina, Birgens, Henrik, Petersen, Jesper Brix
• Format: Journal Article
• Language: English
• Published: Frontiers Media S.A 15.09.2020
• Subjects: anemia; ektacytometry; EMA binding test; hemolysis; hereditary spherocytosis; membranopathy; Physiology

Hereditary spherocytosis (HS) is a common anemia caused by germline mutations in red blood cell cytoskeleton proteins. The flow cytometry-based eosin-5′-maleimide (EMA) binding test is most frequently employed for reliable diagnostics. To perform this test, a number of healthy and ideally also age-m...