Improving the EMA Binding Test by Using Commercially Available Fluorescent Beads
Hereditary spherocytosis (HS) is a common anemia caused by germline mutations in red blood cell cytoskeleton proteins. The flow cytometry-based eosin-5′-maleimide (EMA) binding test is most frequently employed for reliable diagnostics. To perform this test, a number of healthy and ideally also age-m...
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Published in | Frontiers in physiology Vol. 11; p. 569289 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
15.09.2020
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Subjects | |
Online Access | Get full text |
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Summary: | Hereditary spherocytosis (HS) is a common anemia caused by germline mutations in red blood cell cytoskeleton proteins. The flow cytometry-based eosin-5′-maleimide (EMA) binding test is most frequently employed for reliable diagnostics. To perform this test, a number of healthy and ideally also age-matched controls are required, which can be challenging and complicates interlaboratory comparisons. To overcome this limitation, we modified the EMA binding test by replacing healthy controls with commercially available fluorescent beads. Blood samples from 289 individuals with suspected HS were analyzed using the EMA binding test with fluorescent beads and benchmarked against regular EMA binding test using two control samples. Using osmotic gradient ektacytometry as validation, 112 individuals (38.8%) were diagnosed with HS. Performance of the modified EMA binding test was not compromised (accuracy 90.3%) compared to EMA binding test using matched controls (accuracy 88.6%). Based on these findings, we conclude that the modified EMA binding test with fluorescent beads is an attractive alternative, especially in laboratories without easy access to matched controls. Furthermore, as fluorescent beads are stable and easily commutable, they could facilitate both interlaboratory comparisons and quality assessment programs. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Richard Van Wijk, Utrecht University, Netherlands This article was submitted to Red Blood Cell Physiology, a section of the journal Frontiers in Physiology Reviewed by: Satheesh Chonat, Emory University, United States; Elisa Fermo, IRCCS Ca’ Granda Foundation Maggiore Policlinico Hospital, Italy; Rob Van Zwieten, Sanquin Diagnostic Services, Netherlands |
ISSN: | 1664-042X 1664-042X |
DOI: | 10.3389/fphys.2020.569289 |