Efficient Preparation of Chitooligosaccharide With a Potential Chitosanase Csn-SH and Its Application for Fungi Disease Protection
Chitosanase plays a vital role in bioactive chitooligosaccharide preparation. Here, we characterized and prepared a potential GH46 family chitosanase from Bacillus atrophaeus BSS. The purified recombinant enzyme Csn-SH showed a molecular weight of 27.0 kDa. Csn-SH displayed maximal activity toward c...
Saved in:
Published in | Frontiers in microbiology Vol. 12; p. 682829 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
17.06.2021
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Chitosanase plays a vital role in bioactive chitooligosaccharide preparation. Here, we characterized and prepared a potential GH46 family chitosanase from
Bacillus atrophaeus
BSS. The purified recombinant enzyme Csn-SH showed a molecular weight of 27.0 kDa. Csn-SH displayed maximal activity toward chitosan at pH 5.0 and 45°C. Thin-layer chromatography and electrospray ionization–mass spectrometry indicated that Csn-SH mainly hydrolyzed chitosan into (GlcN)
2
, (GlcN)
3
, and (GlcN)
4
with an endo-type cleavage pattern. Molecular docking analysis demonstrated that Csn-SH cleaved the glycoside bonds between subsites −2 and + 1 of (GlcN)
6
. Importantly, the chitosan hydrolysis rate of Csn-SH reached 80.57% within 40 min, which could reduce time and water consumption. The hydrolysates prepared with Csn-SH exhibited a good antifungal activity against
Magnaporthe oryzae
and
Colletotrichum higginsianum
. The above results suggested that Csn-SH could be used to produce active chitooligosaccharides efficiently that are biocontrol agents applicable for safe and sustainable agricultural production. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Microbiotechnology, a section of the journal Frontiers in Microbiology Reviewed by: Abhishek Walia, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya, India; Jagat Rathod, National Cheng Kung University, Taiwan; Ketan D. Patel, University at Buffalo, United States Edited by: Sanket J. Joshi, Sultan Qaboos University, Oman |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2021.682829 |