Identification and Antifungal Susceptibility Analysis of Stephanoascus ciferrii Complex Species Isolated From Patients With Chronic Suppurative Otitis Media
Background Stephanoascus ciferrii is a heterothallic ascomycetous yeast-like fungus. Recently, the concept of S. ciferrii complex has been proposed and it consists of S. ciferrii , Candida allociferrii , and Candida mucifera . We aimed to identify 32 strains of S. ciferrii complex isolated from pati...
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Published in | Frontiers in microbiology Vol. 12; p. 680060 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
21.07.2021
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Subjects | |
Online Access | Get full text |
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Summary: | Background
Stephanoascus ciferrii
is a heterothallic ascomycetous yeast-like fungus. Recently, the concept of
S. ciferrii
complex has been proposed and it consists of
S. ciferrii
,
Candida allociferrii
, and
Candida mucifera
. We aimed to identify 32 strains of
S. ciferrii
complex isolated from patients with chronic suppurative otitis media (CSOM) at the species level and analyze the morphology and antifungal susceptibility profiles of the three species.
Method
The sequencing of the internal transcribed spacer (ITS) region and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used to identify
S. ciferrii
complex species. The SARAMIS software was used for cluster analysis of the mass spectra. All the strains were cultured on Sabouraud dextrose agar (SDA) and CHROM plates for 7 days. In the meantime, colonies of the 32 strains went through Gram staining. The Sensititre YeastOne YO10 colorimetric panel was used for the antifungal susceptibility analysis.
Results
There were 10 strains of
C. allociferrii
(31.25%), six strains of
C. mucifera
(18.75%), and 16 strains of
S. ciferrii
(50%) in the 32 strains of
S. ciferrii
complex according to the sequencing of the ITS region. MALDI-TOF MS could identify
S. ciferrii
but showed no results for
C. allociferrii
and
C. mucifera
. The cluster analysis of the mass spectra by SARAMIS indicated that the MALDI-TOF MS could distinguish the three species. The morphology characteristics of the three species were similar. As for antifungal susceptibility,
S. ciferrii
and
C. mucifera
tended to have high fluconazole MICs compared with
C. allociferrii
.
C. mucifera
and
C. allociferrii
had relatively low flucytosine MICs while
S. ciferrii
owned high flucytosine MICs. Besides,
C. mucifera
tended to have a higher MIC value than
S. ciferrii
for amphotericin B and
C. allociferrii
for anidulafungin, micafungin, and caspofungin.
Conclusion
The antifungal susceptibility profiles of the three species of
S. ciferrii
complex had their own characteristics. Besides, more mass spectra of
C. allociferrii
and
C. mucifera
are needed to construct the reference database for
S. ciferrii
complex species, enabling MALDI-TOF MS to identify
S. ciferrii
complex at species level. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Luciana Lopes Guimaraes, University of Santa Cecília, Brazil; Renata Toth, University of Szeged, Hungary These authors have contributed equally to this work This article was submitted to Fungi and Their Interactions, a section of the journal Frontiers in Microbiology Edited by: Marcos Sergio Toledo, Federal University of São Paulo, Brazil |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2021.680060 |