Is TAP20102 allele involved in insulin-dependent diabetes mellitus (type 1) protection?

• Published in: Human immunology Vol. 65; no. 8; pp. 783 - 793
• Main Authors: Cesari, Maya, Hoarau, Jean-Jacques, Caillens, Henri, Robert, Christine, Rouch, Claude, Cadet, Frederic, Pabion, Michel
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.08.2004
• Subjects: allele protection; amplification refractory mutation system; ARMS; ATP-Binding Cassette Sub-Family B Member 2; ATP-Binding Cassette Transporters - genetics; ATP-Binding Cassette, Sub-Family B, Member 3; Diabetes Mellitus, Type 1 - genetics; Diabetes Mellitus, Type 1 - prevention & control; DNA Fingerprinting; Family Health; Gene Frequency; Genes, MHC Class II; genotyping; haplotype relative risk; Haplotypes - genetics; HLA Antigens - genetics; HLA class II haplotype; HLA-DQ alpha-Chains; HLA-DQ Antigens - genetics; HLA-DQ beta-Chains; HLA-DR Antigens - genetics; HLA-DRB1 Chains; HRR; Humans; IDDM; insulin-dependent diabetes mellitus (type 1); Linkage Disequilibrium; linkage dysequilibrium; TAP; Telomere - genetics; transporter associated with antigen processing; France, La Reunion Island; allele protection; TAP; HLA class II haplotype; IDDM; genotyping; HRR; ARMS; linkage dysequilibrium
• ISSN: 0198-8859; 1879-1166
• DOI: 10.1016/j.humimm.2004.05.013

In this study, we have investigated the frequencies of TAP1 and TAP2 alleles in a group of 226 persons, living in La Reunion Island, consisting of 70 patients with insulin-dependent diabetes mellitus (IDDM) and most of their first degree relatives ( i.e., 156 parents and full sibling subjects) and previously HLA DQB1, DQA1, and DRB1 genotyped. The population of this island is constituted by a particular structure of highly crossbreeding people. Interestingly, the new TAP2*0104 allele, previously discovered by our team in Reunion Island, was found to be increased in the IDDM population and the calculated HRR was relatively high (HRR = 3.3). This result seems to be due to a positive linkage disequilibrium between TAP2*0104 allele and the highly diabetogenous DQB1* 0201–DQA1* 0501–DRB1 0301 haplotype (HRR = 9), which suggests that TAP2*0104 cannot be considered as an additional predispositional factor, but more as a genetic susceptibility marker of IDDM. In addition, we show that minor alleles (TAP2D, *0102, *0103, *0104) are associated with a restricted number of HLA DQ-DR haplotypes and each of them exhibits a preferential linkage with one particular haplotype. In contrast with other alleles, and despite a HRR value close to 1, we show that TAP2*0102 allele contributes significantly to a drastic reduction of the diabetogenic effect of DQB1*0201–DQA1*0301.1–DRB*0701 haplotype. Indeed, this haplotype, which is usually preferentially transmitted to affected children, is dominantly transmitted to healthy children when it is associated with TAP2*0102. Therefore, this allele seems to contribute to genetic protection to IDDM.