The Role of Galanin in Cerebellar Granule Cell Migration in the Early Postnatal Mouse during Normal Development and after Injury

• Published in: The Journal of neuroscience Vol. 41; no. 42; pp. 8725 - 8741
• Main Authors: Komuro, Yutaro, Galas, Ludovic, Morozov, Yury M, Fahrion, Jennifer K, Raoult, Emilie, Lebon, Alexis, Tilot, Amanda K, Kikuchi, Shin, Ohno, Nobuhiko, Vaudry, David, Rakic, Pasko, Komuro, Hitoshi
• Format: Journal Article
• Language: English
• Published: United States Society for Neuroscience 20.10.2021
• Subjects: Animals; Animals, Newborn; Axotomy; Brain; Brain - growth & development; Brain - metabolism; Brain Injuries - metabolism; Brain Injuries - pathology; Brain injury; Calcium ions; Calcium signalling; Cell adhesion & migration; Cell migration; Cell Movement - physiology; Cells, Cultured; Cerebellum; Cerebellum - injuries; Cerebellum - metabolism; Cerebellum - pathology; Dose-Response Relationship, Drug; Epilepsy; Female; Fetuses; Galanin; Galanin - metabolism; Granular materials; Granule cells; Head injuries; Ischemia; Lesions; Male; Mice; Neuropeptides; Signaling; Therapeutic applications; Therapeutic targets; Traumatic brain injury; galanin; live cell imaging; mice; neuronal migration; cerebellum; granule cell
• ISSN: 0270-6474; 1529-2401
• DOI: 10.1523/JNEUROSCI.0900-15.2021

Galanin, one of the most inducible neuropeptides, is widely present in developing brains, and its expression is altered by pathologic events (e.g., epilepsy, ischemia, and axotomy). The roles of galanin in brain development under both normal and pathologic conditions have been hypothesized, but the question of how galanin is involved in fetal and early postnatal brain development remains largely unanswered. In this study, using granule cell migration in the cerebellum of early postnatal mice (both sexes) as a model system, we examined the role of galanin in neuronal cell migration during normal development and after brain injury. Here we show that, during normal development, endogenous galanin participates in accelerating granule cell migration via altering the Ca and cAMP signaling pathways. Upon brain injury induced by the application of cold insults, galanin levels decrease at the lesion sites, but increase in the surroundings of lesion sites. Granule cells exhibit the following corresponding changes in migration: (1) slowing down migration at the lesion sites; and (2) accelerating migration in the surroundings of lesion sites. Experimental manipulations of galanin signaling reduce the lesion site-specific changes in granule cell migration, indicating that galanin plays a role in such deficits in neuronal cell migration. The present study suggests that manipulating galanin signaling may be a potential therapeutic target for acutely injured brains during development. Deficits in neuronal cell migration caused by brain injury result in abnormal development of cortical layers, but the underlying mechanisms remain to be determined. Here, we report that on brain injury, endogenous levels of galanin, a neuropeptide, are altered in a lesion site-specific manner, decreasing at the lesion sites but increasing in the surroundings of lesion sites. The changes in galanin levels positively correlate with the migration rate of immature neurons. Manipulations of galanin signaling ameliorate the effects of injury on neuronal migration and cortical layer development. These results shed a light on galanin as a potential therapeutic target for acutely injured brains during development.