Hepatocyte-specific Deletion of Janus Kinase 2 (JAK2) Protects against Diet-induced Steatohepatitis and Glucose Intolerance

• Published in: The Journal of biological chemistry Vol. 287; no. 13; pp. 10277 - 10288
• Main Authors: Shi, Sally Yu, Martin, Rubén García, Duncan, Robin E., Choi, Diana, Lu, Shun-Yan, Schroer, Stephanie A., Cai, Erica P., Luk, Cynthia T., Hopperton, Kathryn E., Domenichiello, Anthony F., Tang, Christine, Naples, Mark, Dekker, Mark J., Giacca, Adria, Adeli, Khosrow, Wagner, Kay-Uwe, Bazinet, Richard P., Woo, Minna
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 23.03.2012; American Society for Biochemistry and Molecular Biology
• Subjects: Adiposity - drug effects; Adiposity - genetics; Animals; Dietary Fats - adverse effects; Dietary Fats - pharmacology; Fatty Liver - chemically induced; Fatty Liver - enzymology; Fatty Liver - genetics; Fatty Liver - pathology; Gene Deletion; Glucose Intolerance - chemically induced; Glucose Intolerance - enzymology; Glucose Intolerance - genetics; Glucose Intolerance - pathology; Glucose Metabolism; Hepatocytes - enzymology; Hepatocytes - pathology; Inflammation; Insulin Resistance - genetics; Jak Kinase; Janus Kinase 2 - genetics; Janus Kinase 2 - metabolism; Lipid Metabolism; Liver; Metabolic Syndrome; Metabolism; Mice; Mice, Knockout; Non-alcoholic Fatty Liver Disease; Liver; Metabolic Syndrome; Glucose Metabolism; Non-alcoholic Fatty Liver Disease; Lipid Metabolism; Inflammation; Jak Kinase
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M111.317453

Non-alcoholic fatty liver disease (NAFLD) is becoming the leading cause of chronic liver disease and is now considered to be the hepatic manifestation of the metabolic syndrome. However, the role of steatosis per se and the precise factors required in the progression to steatohepatitis or insulin resistance remain elusive. The JAK-STAT pathway is critical in mediating signaling of a wide variety of cytokines and growth factors. Mice with hepatocyte-specific deletion of Janus kinase 2 (L-JAK2 KO mice) develop spontaneous steatosis as early as 2 weeks of age. In this study, we investigated the metabolic consequences of jak2 deletion in response to diet-induced metabolic stress. To our surprise, despite the profound hepatosteatosis, deletion of hepatic jak2 did not sensitize the liver to accelerated inflammatory injury on a prolonged high fat diet (HFD). This was accompanied by complete protection against HFD-induced whole-body insulin resistance and glucose intolerance. Improved glucose-stimulated insulin secretion and an increase in β-cell mass were also present in these mice. Moreover, L-JAK2 KO mice had progressively reduced adiposity in association with blunted hepatic growth hormone signaling. These mice also exhibited increased resting energy expenditure on both chow and high fat diet. In conclusion, our findings indicate a key role of hepatic JAK2 in metabolism such that its absence completely arrests steatohepatitis development and confers protection against diet-induced systemic insulin resistance and glucose intolerance. JAK2 mediates signaling by a number of cytokines in the liver. Hepatic JAK2 KO mice developed spontaneous steatosis but were protected from high fat diet-induced steatohepaitits and insulin resistance. Hepatic JAK2 is required for the development of diet-induced steatohepatitis and glucose intolerance. Understanding the role of JAK2 in metabolism will provide insights into the pathogenesis of the metabolic syndrome.