Characterization of a Mycobacterium smegmatis mutant that is simultaneously resistant to D-cycloserine and vancomycin

A mutant of Mycobacterium smegmatis has been isolated that is simultaneously resistant to both D-cycloserine (D-CS) and vancomycin. Genetic complementation with a PBP4 homolog restores sensitivity to both drugs. Resistance to D-CS and vancomycin in this mutant is most likely due to a novel mechanism...

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Published inAntimicrobial agents and chemotherapy Vol. 44; no. 6; pp. 1701 - 1704
Main Authors Peteroy, M, Severin, A, Zhao, F, Rosner, D, Lopatin, U, Scherman, H, Belanger, A, Harvey, B, Hatfull, G F, Brennan, P J, Connell, N D
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.06.2000
SeriesNote
Subjects
Amino Acid Sequence
Cycloserine - pharmacology
D-Cycloserine
Drug Resistance, Microbial
Mechanisms of Resistance
Molecular Sequence Data
Mutation
Mycobacterium smegmatis
Mycobacterium smegmatis - drug effects
Mycobacterium smegmatis - genetics
PBP4 homolog
PBP4 protein
peptidoglycan assembly
peptidoglycans
Sequence Alignment
Vancomycin - pharmacology
Vancomycin Resistance
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Summary:A mutant of Mycobacterium smegmatis has been isolated that is simultaneously resistant to both D-cycloserine (D-CS) and vancomycin. Genetic complementation with a PBP4 homolog restores sensitivity to both drugs. Resistance to D-CS and vancomycin in this mutant is most likely due to a novel mechanism involving peptidoglycan assembly at the cell surface.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics, UMDNJ/New Jersey Medical School, 185 South Orange Ave., Newark, NJ 07103-2714. Phone: (973) 972-3759. Fax: (973) 972-3644. E-mail: connell@umdnj.edu.
ISSN:0066-4804
1098-6596
DOI:10.1128/AAC.44.6.1701-1704.2000