Effects of Toxic Organotin Compounds on Bacteria Investigated by Micro-Raman Spectroscopy

• Published in: Spectroscopy letters Vol. 41; no. 1; pp. 19 - 28
• Main Authors: Daniel, Ph, Picart, P., Bendriaa, L., Sockalingum, G. D., Adt, I., Charrier, Th, Durand, M. J., Ergan, F., Manfait, M., Thouand, G.
• Format: Journal Article
• Language: English
• Published: Taylor & Francis Group 01.01.2008; Taylor & Francis
• Subjects: Chemical Sciences; Escherichia coli; Escherichia coli TBT3; HCA analysis; organometallic compound; Raman spectroscopy; recombinant bacteria; recombinant bacteria; Escherichia coli TBT3; HCA analysis; Raman spectroscopy; organometallic compound
• ISSN: 0038-7010; 1532-2289
• DOI: 10.1080/00387010701799621

Within the framework of the detection of toxic substances using specific bacteria, the effect of the toxic chemical compound tributyltin chloride (TBT) on the bacterium Escherichia coli TBT3 was investigated using a noninvasive approach based on micro-Raman spectroscopy. Various cellular densities with optical density (OD) ranging from 1 to 4 and harvested at different growth phases were induced for 1 h with different TBT concentrations: 1, 3, 5, and 10 µM. As an original contribution, the Raman results show that, in these conditions, the vibrational signatures of bacteria were affected by the presence of the toxic TBT compound, mainly in the signal characteristics from carbohydrates and lipids. Hierarchical cluster analysis (HCA) carried out on the Raman data in these regions makes it possible to classify the spectra in three groups according to the TBT concentration, confirming the progressive influence of the chemical pollutant on the bacterium. This work shows the potentiality for the Raman technique on this subject, even though a low detection threshold corresponding with a concentration of 1 µM of TBT was established.