Impact of epicatechin on fibrin clot structure

• Published in: European journal of pharmacology Vol. 893; p. 173830
• Main Authors: Sinegre, Thomas, Milenkovic, Dragan, Teissandier, Dorian, Fully, Pauline, Bourdin, Julien, Morand, Christine, Lebreton, Aurélien
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.02.2021; Elsevier
• Subjects: Adult; Blood Coagulation - drug effects; Cardiovascular disease; Catechin - pharmacology; Coagulation; Epicatechin; Female; Fibrin - metabolism; Fibrin - ultrastructure; Fibrin clot; Fibrinolytic Agents - pharmacology; Healthy Volunteers; Humans; Kinetics; Life Sciences; Male; Middle Aged; Permeability; Pharmaceutical sciences; Pharmacology; Turbidity; Young Adult; Cardiovascular disease; Fibrin clot; Permeability; Coagulation; Turbidity; Epicatechin
• ISSN: 0014-2999; 1879-0712; 1879-0712; 0014-2999
• DOI: 10.1016/j.ejphar.2020.173830

Fibrin clot structure and function are major determinants of thromboembolic diseases. The study aim was to determine the impact of epicatechin (a flavonoid with cardiovascular protective effects) on fibrin clot structure and permeability. Plasma samples from 12 healthy subjects were incubated with increasing concentrations of epicatechin. Turbidity of fibrin clot was analyzed by absorbance measurement at 405 nm. The fibrin clot nanostructure was determined by scanning spectrometry (wavelength from 500 to 800 nm) and fibrin fiber size by electron microscopy. Permeability was analyzed to assess the fibrin clot functional properties. Epicatechin addition increased the maximum absorbance from 0.34 ± 0.066 (vehicle) to 0.35 ± 0.077 (P = 0.1), 0.35 ± 0.072 (P < 0.05) and 0.34 ± 0.065 (P = 0.5) for 1, 10 and 100 μM epicatechin, respectively. Epicatechin increased the fibrin clot fiber radius (nm) from 109.2 ± 3.2 (vehicle) to 108.9 ± 4.3 (P = 0.9), 110.0 ± 3.6 (P < 0.05) and 109.5 ± 3.3 (P = 0.4), and the distance between protofibrils (nm) from 22.2 ± 1.5 (vehicle) to 22.1 ± 2.3 (P = 0.9), 22.6 ± 1.8 (P < 0.05) and 22.3 ± 1.8 (P = 0.9) for 1, 10 and 100 μM epicatechin respectively. Electron microscopy confirmed these changes. Fibrin clot permeability, expressed as Darcy's constant (Ks, cm2), increased from 2.97 ± 1.17 (vehicle) to 3.36 ± 1.21 (P < 0.05), 3.81 ± 1.41 (P < 0.01) and 3.38 ± 1.33 (P = 0.9). Upon epicatechin addition, the fibrin clot structure became less dense and more permeable.