Symmetry dual functional pyrimidine-BODIPY probes for imaging targeting and activity study
Nondestructive diagnosis of tumor has always been the goal of scientists. Fluorescent dyes have become the rising star in the field of cancer diagnosis because of their excellent characteristics. Therefore, in this work, fluorescence probes d-Y-B and dO-Y-B with anti-tumor activity were constructed...
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Published in | Frontiers in chemistry Vol. 10; p. 977008 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
20.09.2022
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Subjects | |
Online Access | Get full text |
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Summary: | Nondestructive diagnosis of tumor has always been the goal of scientists. Fluorescent dyes have become the rising star in the field of cancer diagnosis because of their excellent characteristics. Therefore, in this work, fluorescence probes
d-Y-B
and
dO-Y-B
with anti-tumor activity were constructed by introducing pyrimidine groups with high anti-tumor activity using fluorescence dye BODIPY as parent nucleus. The modified BODIPY group in the structure had the advantage of fluorescent dye, ensuring the strong fluorescence and photosensitivity of the target compound. That ethylenediamine acts as a bridge with two -NH- groups to increase molecular hydrogen bonding, and can bind firmly to multiple proteins. Co-localization of the target compounds
d-Y-B
and
dO-Y-B
with the hoechst dye for labeling living cells showed that these compounds had high biocompatibility and photostability for localization to HeLa cells.
In vivo
imaging in mice can realize specific localization and real-time visualization of tumor cells. The results of cytotoxicity experiments
in vitro
and computer software simulating molecular docking confirmed the potential of the target compounds as an anticancer agents. The bifunctional probe realized visualization of cancer cells in mice, and can kill cancer cells by anti-proliferation, which may provide a direction for future anticancer drug development. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Iti Gupta, Indian Institute of Technology Gandhinagar, India Edited by: Pinaki Talukdar, Indian Institute of Science Education and Research, India This article was submitted to Chemical Biology, a section of the journal Frontiers in Chemistry These authors have contributed equally to this work J. Cristobal Lopez, Institute of General Organic Chemistry (CSIC), Spain |
ISSN: | 2296-2646 2296-2646 |
DOI: | 10.3389/fchem.2022.977008 |