Spermidine Is Critical for Growth, Development, Environmental Adaptation, and Virulence in Fusarium graminearum
Putrescine, spermidine, and spermine are the most common natural polyamines. Polyamines are ubiquitous organic cations of low molecular weight and have been well characterized for the cell function and development processes of organisms. However, the physiological functions of polyamines remain larg...
Saved in:
Published in | Frontiers in microbiology Vol. 12; p. 765398 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
19.11.2021
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Putrescine, spermidine, and spermine are the most common natural polyamines. Polyamines are ubiquitous organic cations of low molecular weight and have been well characterized for the cell function and development processes of organisms. However, the physiological functions of polyamines remain largely obscure in plant pathogenic fungi.
Fusarium graminearum
causes Fusarium head blight (FHB) and leads to devastating yield losses and quality reduction by producing various kinds of mycotoxins. Herein, we genetically analyzed the gene function of the polyamine biosynthesis pathway and evaluated the role of the endogenous polyamines in the growth, development, and virulence of
F. graminearum
. Our results found that deletion of spermidine biosynthesis gene
FgSPE3
caused serious growth defects, reduced asexual and sexual reproduction, and increased sensitivity to various stresses. More importantly, Δ
Fgspe3
exhibited significantly decreased mycotoxin deoxynivalenol (DON) production and weak virulence in host plants. Additionally, the growth and virulence defects of Δ
Fgspe3
could be rescued by exogenous application of 5 mM spermidine. Furthermore, RNA-seq displayed that FgSpe3 participated in many essential biological pathways including DNA, RNA, and ribosome synthetic process. To our knowledge, these results indicate that spermidine is essential for growth, development, DON production, and virulence in
Fusarium
species, which provides a potential target to control FHB. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Hai-Lei Wei, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences (CAAS), China Reviewed by: Synan F. AbuQamar, United Arab Emirates University, United Arab Emirates; Zhendong Cai, Ningbo University, China This article was submitted to Microbe and Virus Interactions with Plants, a section of the journal Frontiers in Microbiology |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2021.765398 |