Characterization of Ty3-gypsy-like Elements in Hibiscus syriacus

• Published in: Molecules and cells Vol. 19; no. 3; pp. 318 - 327
• Main Authors: Jeung, J.U. (IRRI-Korea Office, NICS, RDA, Suwon, Republic of Korea), Cho, S.K. (Korea University, Seoul, Republic of Korea), Lee, S.J. (Korea University, Seoul, Republic of Korea), Shin, J.S. (Korea University, Seoul, Republic of Korea), E-mail: jsshin@korea.ac.kr
• Format: Journal Article
• Language: English
• Published: United States 30.06.2005
• Subjects: Amino Acid Sequence; Base Sequence; Genes, Plant - genetics; Genetic Diversity; Genome, Plant; gypsy-like Retrotransposon; Hibiscus - classification; Hibiscus - genetics; HIBISCUS SYRIACUS; Molecular Sequence Data; ntegrase; Nucleic Acid Hybridization; Phylogenetic Analysis; Polymorphism, Restriction Fragment Length; Retroelements - genetics; Sequence Alignment; Sequence Tagged Sites
• ISSN: 1016-8478
• DOI: 10.1016/s1016-8478(23)13175-x

Southern blot analysis revealed a ubiquitous distribu-tion and high copy number of Ty3-gypsy-like elements in the genome of Hibiscus syriacus. Comparative phylogenetic analysis of the large subunit of Rubisco and the integrase region of Ty3-gypsy elements in various plant species indicated that the retrotransposon-like sequences have different evolutionary histories and their own unique polymorphism in the H. syriacus population. Sequence-tagged site-restriction fragment length polymorphisms (STS-RFLP) analysis also indicated great variability in the numbers and sequences of Ty3-gypsy-like elements within H. syriacus varieties. Ty3-gypsy-like elements may still be active within H. syriacus, since Northern analysis of wounded leaves of H. syriacus variety Saehan with a probe for the integrase domain gave strong hybridization signals.