Sensitive and specific morphine radioimmunoassay with iodine label: pharmacokinetics of morphine in man after intravenous administration

Analysis of morphine in plasma by radioimmunoassay is complicated by interference from morphine metabolites, particularly morphine-3-glucuronide. This interference makes radioimmunoassay a poor technique to study the pharmacokinetics of morphine in man. The method described here is one which uses co...

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Bibliographic Details
Published inAnnals of clinical biochemistry Vol. 21 ( Pt 4); p. 318
Main Authors Moore, R A, Baldwin, D, Allen, M C, Watson, P J, Bullingham, R E, McQuay, H J
Format Journal Article
LanguageEnglish
Published England 01.07.1984
Subjects
Chromatography, High Pressure Liquid
Cross Reactions
Humans
Injections, Intravenous
Iodine Radioisotopes
Kinetics
Laminectomy
Middle Aged
Morphine - administration & dosage
Morphine - blood
Radioimmunoassay - methods
Time Factors
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Summary:Analysis of morphine in plasma by radioimmunoassay is complicated by interference from morphine metabolites, particularly morphine-3-glucuronide. This interference makes radioimmunoassay a poor technique to study the pharmacokinetics of morphine in man. The method described here is one which uses commercially available anti-morphine antiserum and separating reagent with a simply prepared iodinated morphine derivative. Cross-reactivity to morphine metabolites is negligible and the method correlates well with HPLC. Results obtained for pharmacokinetic parameters after intravenous administration in man are in agreement with published data using GLC or HPLC. The method is inexpensive, simple and rapid; choice of a different antiserum could allow the method to be used for screening for drugs of abuse such as morphine, diamorphine, codeine and dihydrocodeine.
ISSN:0004-5632
DOI:10.1177/000456328402100415