Influence of lignosulfonate on crystal structure and productivity of bacterial cellulose in a static culture

• Published in: Enzyme and microbial technology Vol. 40; no. 1; pp. 4 - 8
• Main Authors: Keshk, S., Sameshima, K.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.12.2006; Elsevier Science
• Subjects: Bacteria; Bacterial cellulose; Biological and medical sciences; Biotechnology; CP/MAS 13C NMR spectroscopy; FT-IR; Fundamental and applied biological sciences. Psychology; Gluconacetobacter xylinus; Lignosulfonate; X-ray diffractogram; Bacterial cellulose; FT-IR; X-ray diffractogram; CP/MAS 13C NMR spectroscopy; Lignosulfonate; Gluconacetobacter xylinus; Productivity; Fourier transformation; Cellulose; Crystals; X ray; NMR spectrometry; Infrared spectrometry; Bacteria; Culture
• ISSN: 0141-0229; 1879-0909
• DOI: 10.1016/j.enzmictec.2006.07.037

The influence of lignosulfonate on bacterial cellulose (BC) structure and productivity was studied using six strains of Gluconacetobacter xylinus (= Acetobacter xylinum) (ATCC 10245, IFO 13693, 13772, 13773, 14815 and 15237). The BC productivity of all strains was improved almost 57% in the presence of (1%, w/v) lignosulfonate. FT-IR result showed that the BC produced in the presence of lignosulfonate displayed the higher crystallinity index and I α-rich cellulose. Furthermore, X-ray diffractogram confirmed the FT-IR results and showed no remarkable change in d-spacing. From CP/MAS 13C NMR spectra, the amorphous region in the presence of lignosulfonate was relatively lower, that means the lignosulfonate enhanced the crystallinity of BC. These results indicate that the increase in the BC yield is due to the inhibition of gluconic acid formation in the presence of the antioxidant, polyphenolic compounds in the lignosulfonate.