Engineered Remolding and Application of Bacterial Membrane Vesicles

Bacterial membrane vesicles (MVs) are produced by both Gram-positive and Gram-negative bacteria during growth in vitro and in vivo . MVs are nanoscale vesicular structures with diameters ranging from 20 to 400 nm. MVs incorporate bacterial lipids, proteins, and often nucleic acids, and can effective...

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Published inFrontiers in microbiology Vol. 12; p. 729369
Main Authors Qiao, Li, Rao, Yifan, Zhu, Keting, Rao, Xiancai, Zhou, Renjie
Format Journal Article
LanguageEnglish
Published Frontiers Media S.A 08.10.2021
Subjects
delivery system
extracellular vesicles
genetic modification
Microbiology
vaccine
vesicle immunogenicity
vesicle production
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Summary:Bacterial membrane vesicles (MVs) are produced by both Gram-positive and Gram-negative bacteria during growth in vitro and in vivo . MVs are nanoscale vesicular structures with diameters ranging from 20 to 400 nm. MVs incorporate bacterial lipids, proteins, and often nucleic acids, and can effectively stimulate host immune response against bacterial infections. As vaccine candidates and drug delivery systems, MVs possess high biosafety owing to the lack of self-replication ability. However, wild-type bacterial strains have poor MV yield, and MVs from the wild-type strains may be harmful due to the carriage of toxic components, such as lipopolysaccharides, hemolysins, enzymes, etc. In this review, we summarize the genetic modification of vesicle-producing bacteria to reduce MV toxicity, enhance vesicle immunogenicity, and increase vesicle production. The engineered MVs exhibit broad applications in vaccine designs, vaccine delivery vesicles, and drug delivery systems.
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Reviewed by: Mariola J. Edelmann, University of Florida, United States; Susanne Erdmann, Max Planck Institute for Marine Microbiology (MPG), Germany
These authors have contributed equally to this work
This article was submitted to Microbiotechnology, a section of the journal Frontiers in Microbiology
Edited by: Elisa Michelini, University of Bologna, Italy
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2021.729369