Selenium-enriched Bifidobacterium longum DD98 effectively ameliorates dextran sulfate sodium-induced ulcerative colitis in mice
The pathogenesis of ulcerative colitis (UC) is complicated with impaired intestinal epithelial barrier and imbalanced gut microbiota. Both selenium and probiotics have shown effects in regulating intestinal flora and ameliorating UC. The objective of this study is to investigate the alleviating effe...
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Published in | Frontiers in microbiology Vol. 13; p. 955112 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
05.08.2022
|
Subjects | |
Online Access | Get full text |
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Summary: | The pathogenesis of ulcerative colitis (UC) is complicated with impaired intestinal epithelial barrier and imbalanced gut microbiota. Both selenium and probiotics have shown effects in regulating intestinal flora and ameliorating UC. The objective of this study is to investigate the alleviating effects of Selenium-enriched
Bifidobacterium longum
DD98 (Se-
B. longum
DD98) on dextran sulfate sodium (DSS)-induced colitis in mice and explore the underlying mechanism. After treatment of
B. longum
DD98, Se-
B. longum
DD98, and sulfasalazine for 3 weeks, the disease severity of UC mice was decreased, with colon lengthened and pathological phenotype improved. The expression of pro-inflammatory cytokines and oxidative stress parameters were also decreased. Thus, Se-
B. longum
DD98 showed a stronger effect on relieving the aforementioned symptoms caused by DSS-induced colitis. Exploration of the potential mechanism demonstrated that Se-
B. longum
DD98 showed higher activities to suppress the inflammatory response by inhibiting the activation of the toll-like receptor 4 (TLR4), compared to
B. longum
DD98 and sulfasalazine. Se-
B. longum
DD98 also significantly improved the intestinal barrier integrity by increasing the expression of tight junction proteins including ZO-1 and occludin. 16S rDNA sequencing analyses showed that Se-
B. longum
DD98 improved the diversity of the intestinal flora and promoted the abundance of health-benefiting taxa including
Lachnospiraceae
,
Lactobacillaceae
, and
Prevotellaceae
in family level. In conclusion, compared to
B. longum
DD98 and sulfasalazine, Se-
B. longum
DD98 showed stronger therapeutic effects on DSS-induced colitis in mice and might be a promising candidate for the treatment of UC. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Rina Wu, Shenyang Agricultural University, China Reviewed by: Sabine Stegemann-Koniszewski, University Hospital Magdeburg, Germany; Yuying Li, Institute of Subtropical Agriculture (CAS), China This article was submitted to Food Microbiology, a section of the journal Frontiers in Microbiology |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2022.955112 |