Enhancement of drought tolerance in Arabidopsis plants induced by sulfur dioxide

• Published in: Ecotoxicology (London) Vol. 31; no. 4; pp. 637 - 648
• Main Authors: Li, Lijuan, Yi, Huilan
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.05.2022; Springer; Springer Nature B.V
• Subjects: Air pollution; Antioxidants; Arabidopsis; Arabidopsis thaliana; Assimilation; Biological products; Drought; Drought resistance; Droughts; Earth and Environmental Science; Ecology; Ecotoxicology; Environment; Environmental Management; Environmental stress; Enzymatic activity; Enzyme activity; Enzymes; Ethylenediaminetetraacetic acid; Fumigation; Gene expression; Glutathione; Hydrogen peroxide; Peroxidase; Photosynthesis; Photosynthetic pigments; Plants; Pollutants; Proline; Proline dehydrogenase; Stomata; Stomatal conductance; Sulfur; Sulfur dioxide; Sulphur; Sulphur dioxide; Superoxide; Superoxide dismutase; Thiols; Transpiration; Water loss; Water use; Water use efficiency; Sulfur assimilation; Photosynthesis; Drought tolerance; Osmotic adjustment; Sulfur dioxide
• ISSN: 0963-9292; 1573-3017
• DOI: 10.1007/s10646-022-02530-w

Sulfur dioxide (SO 2 ) is a common air pollutant that has multiple effects on plants. In the present study, the improvement of drought tolerance in Arabidopsis plants by SO 2 fumigation was investigated. The results showed that pre-exposure to 30 mg/m 3 SO 2 for 72 h could reduce water loss, stomatal conductance (Gs) and the transpiration rate (Tr) but increased the net photosynthetic rate (Pn), water use efficiency (iWUE) and photosynthetic pigment contents under drought conditions. The activities of superoxide dismutase (SOD) and peroxidase (POD) were significantly increased, while the contents of hydrogen peroxide (H 2 O 2 ) and malondialdehyde (MDA) were decreased in SO 2 -pretreated Arabidopsis plants under drought stress. Additionally, the activity of o-acetylserine(thio)lyase (OASTL) and the content of cysteine (Cys), the rate-limiting enzyme and the first organic product of sulfur assimilation, were significantly increased in drought-stressed plants after SO 2 pretreatment, along with increases in other thiol-containing compounds, such as glutathione (GSH) and nonprotein thiol (NPT). Meanwhile, SO 2 pre-exposure induced a higher level of proline accumulation, with increased activity of proline synthase P5CS and decreased activity of proline dehydrogenase ProDH. Consistent with the changes in enzyme activity, their corresponding gene expression patterns were different after SO 2 treatment. Overall, the enhanced drought tolerance afforded by SO 2 might be related to the improvement of plant photosynthesis, antioxidant defense, sulfur assimilation and osmotic adjustment. These findings provide new insights into the role of SO 2 in plant adaptation to environmental stress.