Cocoa intake attenuates oxidative stress associated with rat adjuvant arthritis

Cocoa contains flavonoids with antioxidant properties. The aim of this study was to ascertain the effect of cocoa intake on oxidative stress associated with a model of chronic inflammation such as adjuvant arthritis. Female Wistar rats were fed with a 5% or 10% cocoa-enriched diet or were given p.o....

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Published inPharmacological research Vol. 66; no. 3; pp. 207 - 212
Main Authors Ramos-Romero, S., Pérez-Cano, F.J., Ramiro-Puig, E., Franch, A., Castell, M.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier Ltd 01.09.2012
Elsevier B.V
Subjects
Adjuvant arthritis
Animals
Antioxidants - pharmacology
Arthritis
Arthritis, Experimental - diet therapy
Arthritis, Experimental - metabolism
Artritis
Cacao
Cacau
Catalase - metabolism
Cocoa
Diet - methods
Estrès oxidatiu
Female
Inflammation - drug therapy
Inflammation - metabolism
Macrophages - drug effects
Macrophages - metabolism
Oxidative stress
Oxidative Stress - drug effects
Quercetin - pharmacology
Rats
Rats, Wistar
Reactive Oxygen Species - metabolism
Superoxide Dismutase - metabolism
AA
Oxidative stress
C10
AIN
SOD
H2DCF-DA
Cocoa
EGTA
RA
Adjuvant arthritis
p.o
Q
C5
REF
TNF-α
RNS
ROS
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Summary:Cocoa contains flavonoids with antioxidant properties. The aim of this study was to ascertain the effect of cocoa intake on oxidative stress associated with a model of chronic inflammation such as adjuvant arthritis. Female Wistar rats were fed with a 5% or 10% cocoa-enriched diet or were given p.o. a quercetin suspension every other day for 10days. Arthritis was induced by a heat-killed Mycobacterium butyricum suspension. Reactive oxygen species (ROS) produced by macrophages, and splenic superoxide dismutase (total, cytoplasmic and mitochondrial) and catalase activities were determined. Clinically, joint swelling in arthritic rats was not reduced by antioxidants; however, the 5% cocoa diet and quercetin administration reduced ROS production. Moreover, the 5% cocoa diet normalized the activities of superoxide dismutase and catalase. In conclusion, a cocoa diet reduces the oxidative stress associated with a chronic inflammatory pathology, although it was not enough to attenuate joint swelling.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1043-6618
1096-1186
DOI:10.1016/j.phrs.2012.05.009