Using an untargeted metabolomics approach to analyze serum metabolites in COVID-19 patients with nucleic acid turning negative
Background: The coronavirus disease of 2019 (COVID-19) is a severe public health issue that has infected millions of people. The effective prevention and control of COVID-19 has resulted in a considerable increase in the number of cured cases. However, little research has been done on a complete met...
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Published in | Frontiers in pharmacology Vol. 13; p. 964037 |
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Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
24.08.2022
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Subjects | |
Online Access | Get full text |
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Summary: | Background:
The coronavirus disease of 2019 (COVID-19) is a severe public health issue that has infected millions of people. The effective prevention and control of COVID-19 has resulted in a considerable increase in the number of cured cases. However, little research has been done on a complete metabonomic examination of metabolic alterations in COVID-19 patients following treatment. The current project pursues rigorously to characterize the variation of serum metabolites between healthy controls and COVID-19 patients with nucleic acid turning negative
via
untargeted metabolomics.
Methods:
The metabolic difference between 20 COVID-19 patients (CT ≥ 35) and 20 healthy controls were investigated utilizing untargeted metabolomics analysis employing High-resolution UHPLC-MS/MS. COVID-19 patients’ fundamental clinical indicators, as well as health controls, were also collected.
Results:
Out of the 714 metabolites identified, 203 still significantly differed between COVID-19 patients and healthy controls, including multiple amino acids, fatty acids, and glycerophospholipids. The clinical indexes including monocytes, lymphocytes, albumin concentration, total bilirubin and direct bilirubin have also differed between our two groups of participators.
Conclusion:
Our results clearly showed that in COVID-19 patients with nucleic acid turning negative, their metabolism was still dysregulated in amino acid metabolism and lipid metabolism, which could be the mechanism of long-COVID and calls for specific post-treatment care to help COVID-19 patients recover. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Pharmacology of Infectious Diseases, a section of the journal Frontiers in Pharmacology He Song, University of California, Los Angeles, United States These authors have contributed equally to this work Yijun Shen, Fudan University, China Reviewed by: Huan Wu, Anhui University of Chinese Medicine, China Edited by: Fan Yang, Stanford University, United States |
ISSN: | 1663-9812 1663-9812 |
DOI: | 10.3389/fphar.2022.964037 |