Nutrient Recovery of Starch Processing Waste to Cordyceps militaris: Solid State Cultivation and Submerged Liquid Cultivation

• Published in: Applied biochemistry and biotechnology Vol. 180; no. 2; pp. 274 - 288
• Main Authors: Lee, Joonyeob, Cho, Kyungjin, Shin, Seung Gu, Bae, Hyokwan, Koo, Taewoan, Han, Gyuseong, Hwang, Seokhwan
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.09.2016; Springer Nature B.V
• Subjects: Analysis of Variance; Biochemistry; Biotechnology; Biotechnology - methods; Chemistry; Chemistry and Materials Science; Cordyceps - growth & development; Cordyceps - metabolism; Cultivation; Fungi; Mycelium - growth & development; Mycelium - metabolism; Nitrogen - isolation & purification; Nutrients; Phosphorus - isolation & purification; Polymerase chain reaction; Real-Time Polymerase Chain Reaction; RNA, Ribosomal, 18S - genetics; Starch; Starch - metabolism; Surface chemistry; Temperature; Waste Products; Bioconversion; Response surface analysis (RSA); Fungal process; Real-time quantitative polymerase chain reaction (QPCR); Cordyceps militaris
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-016-2098-4

This study demonstrated the potential for managing starch processing waste (SPW) by bioconversion to Cordyceps militaris mycelia using solid state cultivation (SSC) and submerged liquid cultivation (SLC). The growth characteristics of C. militaris mycelium were accessed and compared for SSC and SLC systems on SPW under various conditions of initial SPW concentration, pH, and operating temperature. To quantify the mycelial biomass in SLC, original primer sets targeting the 18S rRNA gene of C. militaris were developed. In SSC, a maximum mycelial growth rate (543.1 mm 2 /day) was predicted to occur at 25.6 g SPW/L, pH 5.5, and 23.8 °C. In SLC, a maximum mycelial growth rate (1918.6 mg/L/day) was predicted to occur at 35.5 g SPW/L, pH 5.5, and 22.0 °C. Temperature was suggested as the most significant factor in both systems. The higher optimum substrate concentration observed for SLC than for SSC was likely due to difference in mycelial morphology and mixing effect.