Differential modulation of bradykinin-induced relaxation of endothelin-1 and phenylephrine contractions of rat aorta by antioxidants
Aim: We tested the hypothesis that bradykinin (BK)-induced relaxation of phenylephrine (PE) and endothelin-1 (ET-1) contractions can be differentially modu- lated by reactive oxygen species (ROS). Methods: Aortic rings isolated from Sprague-Dawley rats were used for the study. The contribution of RO...
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Published in | Acta pharmacologica Sinica Vol. 28; no. 10; pp. 1566 - 1572 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
Nature Publishing Group
01.10.2007
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Subjects | |
Online Access | Get full text |
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Summary: | Aim: We tested the hypothesis that bradykinin (BK)-induced relaxation of phenylephrine (PE) and endothelin-1 (ET-1) contractions can be differentially modu- lated by reactive oxygen species (ROS). Methods: Aortic rings isolated from Sprague-Dawley rats were used for the study. The contribution of ROS to PE (1× 10^-9-1× 10^5 mol/L)- and ET-1 (1× 10^-10 -1× 10^-8 mol/L)-induced contractions and the influence of ROS in BK (1× 10^-9 - 1× 10^-5 mol/L) relaxation of PE (1× 10^-7 mol/L) or ET-1 (1×10^-9 mol/L)-induced tension was evaluated in the aorta in the presence or absence of the following antioxidants: catalase (CAT, 300 U/mL), superoxide dismutase (SOD, 300 U/mL), and vitamin C (1× 10^-4 mol/L). Results: Tension generated by ET- 1 (1 × 10^-9 mol/L) or PE (1 × 10^-7 mol/L) was differentially relaxed by BK (1 × 10^-5 mol/L), producing a maximal relaxation of 75%±5% and 35±4%, respectively. The BK (1 × 10^-5 mol/L)-induced relaxation of PE (1 × 10^-7 mol/L) tension was significantly enhanced from 35%±4% (control) to 56%±9%, 60%±5%, and 49%±6% by SOD, CAT, and vitamin C, respectively (P〈0.05, n=8). However, the relaxation of ET-1 (1× 10^-9 mol/L) tension was significantly attenuated from 75%±5% (control) to 37%±9%, 63%±4%, and 39%±7% by SOD, CAT, and vitamin C, respectively (P〈0.05, n=8). On the other hand, CAT had no effect on PE-induced tension, while SOD enhanced PE-induced tension (36%, P〈0.05, n=10) and vitamin C attenuated (66%, P〈0.05, n=8) the tension induced by PE. By contrast, SOD or vitamin C had no effect, but CAT attenuated (44%, P〈0.05, n=9) the tension induced by ET-1. Conclusion: We have demonstrated that O2^- and H2O2 differentially modulate BK relaxation in an agonist-specific manner. O2^- attenuates BK-induced relaxation of PE contraction, but contributes to the relaxation of ET- 1 contraction. O2^- seems to inhibit PE contraction, while H2O2 contributes to ET-1-induced contraction. Thus, ROS differentially modulate vascular tone depending on the vasoactive agent that is used to generate the tone. |
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Bibliography: | hydrogenperoxide bradykinin; endothelin- 1 ; phenylephrine;reactive oxygen species; superoxide; hydrogenperoxide; relaxation; contraction; antioxidants antioxidants endothelin- 1 phenylephrine reactive oxygen species relaxation bradykinin contraction superoxide R96 31-1347/R ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1671-4083 1745-7254 |
DOI: | 10.1111/j.1745-7254.2007.00631.x |