Expression Profiles and Functional Characterization of Chemosensory Protein 15 (HhalCSP15) in the Brown Marmorated Stink Bug Halyomorpha halys

Chemosensory proteins (CSPs) have been identified in the sensory tissues of various insect species and are believed to be involved in chemical communication in insects. However, the physiological roles of CSPs in Halyomorpha halys , a highly invasive insect species, are rarely reported. Here, we foc...

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Published inFrontiers in physiology Vol. 12; p. 721247
Main Authors Wang, Zehua, Yang, Fan, Sun, Ang, Shan, Shuang, Zhang, Yongjun, Wang, Shanning
Format Journal Article
LanguageEnglish
Published Frontiers Media S.A 06.09.2021
Subjects
chemosensory protein
electroantennography
expression profile
fluorescence binding assay
Halyomorpha halys
molecular docking
Physiology
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Abstract Chemosensory proteins (CSPs) have been identified in the sensory tissues of various insect species and are believed to be involved in chemical communication in insects. However, the physiological roles of CSPs in Halyomorpha halys , a highly invasive insect species, are rarely reported. Here, we focused on one of the antennal CSPs ( HhalCSP15 ) and determined whether it was involved in olfactory perception. Reverse transcription PCR (RT-PCR) and quantitative real-time PCR (qRT-PCR) analysis showed that HhalCSP15 was enriched in nymph and male and female adult antennae, indicating its possible involvement in the chemosensory process. Fluorescence competitive binding assays revealed that three of 43 natural compounds showed binding abilities with HhalCSP15, including β-ionone ( K i =11.9±0.6μM), cis -3-hexen-1-yl benzoate ( K i =10.5±0.4μM), and methyl (2 E ,4 E ,6 Z )-decatrienoate ( EEZ -MDT; K i =9.6±0.8μM). Docking analysis supported the experimental affinity for the three ligands. Additionally, the electrophysiological activities of the three ligands were further confirmed using electroantennography (EAG). EEZ -MDT is particularly interesting, as it serves as a kairomone when H. halys forages for host plants. We therefore conclude that HhalCSP15 might be involved in the detection of host-related volatiles. Our data provide a basis for further investigation of the physiological roles of CSPs in H. halys , and extend the olfactory function of CSPs in stink bugs.
AbstractList Chemosensory proteins (CSPs) have been identified in the sensory tissues of various insect species and are believed to be involved in chemical communication in insects. However, the physiological roles of CSPs in Halyomorpha halys , a highly invasive insect species, are rarely reported. Here, we focused on one of the antennal CSPs ( HhalCSP15 ) and determined whether it was involved in olfactory perception. Reverse transcription PCR (RT-PCR) and quantitative real-time PCR (qRT-PCR) analysis showed that HhalCSP15 was enriched in nymph and male and female adult antennae, indicating its possible involvement in the chemosensory process. Fluorescence competitive binding assays revealed that three of 43 natural compounds showed binding abilities with HhalCSP15, including β-ionone ( K i =11.9±0.6μM), cis -3-hexen-1-yl benzoate ( K i =10.5±0.4μM), and methyl (2 E ,4 E ,6 Z )-decatrienoate ( EEZ -MDT; K i =9.6±0.8μM). Docking analysis supported the experimental affinity for the three ligands. Additionally, the electrophysiological activities of the three ligands were further confirmed using electroantennography (EAG). EEZ -MDT is particularly interesting, as it serves as a kairomone when H. halys forages for host plants. We therefore conclude that HhalCSP15 might be involved in the detection of host-related volatiles. Our data provide a basis for further investigation of the physiological roles of CSPs in H. halys , and extend the olfactory function of CSPs in stink bugs.
Chemosensory proteins (CSPs) have been identified in the sensory tissues of various insect species and are believed to be involved in chemical communication in insects. However, the physiological roles of CSPs in Halyomorpha halys, a highly invasive insect species, are rarely reported. Here, we focused on one of the antennal CSPs (HhalCSP15) and determined whether it was involved in olfactory perception. Reverse transcription PCR (RT-PCR) and quantitative real-time PCR (qRT-PCR) analysis showed that HhalCSP15 was enriched in nymph and male and female adult antennae, indicating its possible involvement in the chemosensory process. Fluorescence competitive binding assays revealed that three of 43 natural compounds showed binding abilities with HhalCSP15, including β-ionone (Ki=11.9±0.6μM), cis-3-hexen-1-yl benzoate (Ki=10.5±0.4μM), and methyl (2E,4E,6Z)-decatrienoate (EEZ-MDT; Ki=9.6±0.8μM). Docking analysis supported the experimental affinity for the three ligands. Additionally, the electrophysiological activities of the three ligands were further confirmed using electroantennography (EAG). EEZ-MDT is particularly interesting, as it serves as a kairomone when H. halys forages for host plants. We therefore conclude that HhalCSP15 might be involved in the detection of host-related volatiles. Our data provide a basis for further investigation of the physiological roles of CSPs in H. halys, and extend the olfactory function of CSPs in stink bugs.
Author Sun, Ang
Wang, Shanning
Shan, Shuang
Zhang, Yongjun
Wang, Zehua
Yang, Fan
AuthorAffiliation 1 Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences , Beijing , China
2 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences , Beijing , China
AuthorAffiliation_xml – name: 1 Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences , Beijing , China
– name: 2 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences , Beijing , China
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This article was submitted to Invertebrate Physiology, a section of the journal Frontiers in Physiology
Edited by: Jin Zhang, Max Planck Institute for Chemical Ecology, Germany
Reviewed by: Herbert Venthur, University of La Frontera, Chile; Youssef Dewer, Agricultural Research Center, Egypt
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Snippet Chemosensory proteins (CSPs) have been identified in the sensory tissues of various insect species and are believed to be involved in chemical communication in...
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SubjectTerms chemosensory protein
electroantennography
expression profile
fluorescence binding assay
Halyomorpha halys
molecular docking
Physiology
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Title Expression Profiles and Functional Characterization of Chemosensory Protein 15 (HhalCSP15) in the Brown Marmorated Stink Bug Halyomorpha halys
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https://pubmed.ncbi.nlm.nih.gov/PMC8450399
https://doaj.org/article/a4c43488d0124584a390288634dceb80
Volume 12
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