Expression Profiles and Functional Characterization of Chemosensory Protein 15 (HhalCSP15) in the Brown Marmorated Stink Bug Halyomorpha halys
Chemosensory proteins (CSPs) have been identified in the sensory tissues of various insect species and are believed to be involved in chemical communication in insects. However, the physiological roles of CSPs in Halyomorpha halys , a highly invasive insect species, are rarely reported. Here, we foc...
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Published in | Frontiers in physiology Vol. 12; p. 721247 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
06.09.2021
|
Subjects | |
Online Access | Get full text |
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Summary: | Chemosensory proteins (CSPs) have been identified in the sensory tissues of various insect species and are believed to be involved in chemical communication in insects. However, the physiological roles of CSPs in
Halyomorpha halys
, a highly invasive insect species, are rarely reported. Here, we focused on one of the antennal CSPs (
HhalCSP15
) and determined whether it was involved in olfactory perception. Reverse transcription PCR (RT-PCR) and quantitative real-time PCR (qRT-PCR) analysis showed that
HhalCSP15
was enriched in nymph and male and female adult antennae, indicating its possible involvement in the chemosensory process. Fluorescence competitive binding assays revealed that three of 43 natural compounds showed binding abilities with HhalCSP15, including β-ionone (
K
i
=11.9±0.6μM),
cis
-3-hexen-1-yl benzoate (
K
i
=10.5±0.4μM), and methyl (2
E
,4
E
,6
Z
)-decatrienoate (
EEZ
-MDT;
K
i
=9.6±0.8μM). Docking analysis supported the experimental affinity for the three ligands. Additionally, the electrophysiological activities of the three ligands were further confirmed using electroantennography (EAG).
EEZ
-MDT is particularly interesting, as it serves as a kairomone when
H. halys
forages for host plants. We therefore conclude that HhalCSP15 might be involved in the detection of host-related volatiles. Our data provide a basis for further investigation of the physiological roles of CSPs in
H. halys
, and extend the olfactory function of CSPs in stink bugs. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Invertebrate Physiology, a section of the journal Frontiers in Physiology Edited by: Jin Zhang, Max Planck Institute for Chemical Ecology, Germany Reviewed by: Herbert Venthur, University of La Frontera, Chile; Youssef Dewer, Agricultural Research Center, Egypt |
ISSN: | 1664-042X 1664-042X |
DOI: | 10.3389/fphys.2021.721247 |