Genome-Wide Investigation of SBT Family Genes in Pineapple and Functional Analysis of AcoSBT1.12 in Floral Transition
SBT (Subtilisin-like serine protease), a clan of serine proteolytic enzymes, plays a versatile role in plant growth and defense. Although SBT family genes have been obtained from studies of dicots such as Arabidopsis, little is known about the potential functions of SBT in the monocots. In this stud...
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Published in | Frontiers in genetics Vol. 12; p. 730821 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
07.09.2021
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Subjects | |
Online Access | Get full text |
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Summary: | SBT (Subtilisin-like serine protease), a clan of serine proteolytic enzymes, plays a versatile role in plant growth and defense. Although
SBT
family genes have been obtained from studies of dicots such as Arabidopsis, little is known about the potential functions of SBT in the monocots. In this study, 54 pineapple
SBT
genes (
AcoSBT
s) were divided into six subfamilies and then identified to be experienced strong purifying selective pressure and distributed on 25 chromosomes unevenly.
Cis
-acting element analysis indicated that almost all
AcoSBT
s promoters contain light-responsive elements. Further, the expression pattern via RNA-seq data showed that different
AcoSBT
s were preferentially expressed in different above-ground tissues. Transient expression in tobacco showed that AcoSBT1.12 was located in the plasma membrane. Moreover, Transgenic Arabidopsis ectopically overexpressing
AcoSBT1.12
exhibited delayed flowering time. In addition, under the guidance of bioinformatic prediction, we found that AcoSBT1.12 could interact with AcoCWF19L, AcoPUF2, AcoCwfJL, Aco012905, and AcoSZF1 by yeast-two hybrid (Y2H). In summary, this study provided valuable information on pineapple
SBT
genes and illuminated the biological function of
AcoSBT1.12
in floral transition. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Jian Wu, Yangzhou University, China; Xiumei Zhang, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, China These authors have contributed equally to this work Edited by: Zefeng Yang, Yangzhou University, China This article was submitted to Plant Genomics, a section of the journal Frontiers in Genetics |
ISSN: | 1664-8021 1664-8021 |
DOI: | 10.3389/fgene.2021.730821 |