Effective bioremoval and detoxification of textile dye mixture by Alishewanella sp. KMK6

• Published in: Applied microbiology and biotechnology Vol. 97; no. 2; pp. 881 - 889
• Main Authors: Kolekar, Yogesh M., Konde, Pallavi D., Markad, Vijay L., Kulkarni, Sharwari V., Chaudhari, Ashvini U., Kodam, Kisan M.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 2013; Springer Nature B.V
• Subjects: Alteromonadaceae - metabolism; Bacteria; Biodegradation; Biomedical and Life Sciences; Bioremediation; Biotechnology; Carbon; Carbon - metabolism; Carcinogens; Chemical oxygen demand; Chromatography, High Pressure Liquid; Coloring Agents; Dyes; Environmental Biotechnology; Enzymes; Fourier transforms; Life Sciences; Microbial Genetics and Genomics; Microbiology; NADH, NADPH Oxidoreductases - metabolism; Nitrogen; Nitrogen - metabolism; Spectroscopy, Fourier Transform Infrared; Studies; Textiles; Water pollution; Water treatment; Biodegradation; COD; sp. KMK6; Azo dyes; Azoreductase
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-012-3983-6

Alishewanella sp. strain KMK6 was able to degrade mixture of textile dyes (0.5–2.0 g l −1 ) within 8 h. An initial 28 % reduction in COD was observed immediately after decolorization at static anoxic conditions which on further incubation at shaking conditions reduced by 90 %. Partially purified azoreductase was able to utilize different azo dyes as substrates. The HPLC profile of dye degradation showed formation of metabolic products. Further FTIR analysis showed significant changes in the peaks corresponding to functional groups present in dye mixture and its degradation products. The genotoxicity assessment showed that the dye degradation products were non-toxic compared to dye mixture.