Transcriptomic Analysis of Genes Modulated by Cyclo(L-Phenylalanine- L-Proline) in Vibrio vulnificus

• Published in: Journal of microbiology and biotechnology Vol. 23; no. 12; pp. 1791 - 1801
• Main Authors: Kim, I.H., Sogang University, Seoul, Republic of Korea, Son, J.S., Sogang University, Seoul, Republic of Korea, Wen, Y.C., Sogang University, Seoul, Republic of Korea, Jeong, S.M., Sogang University, Seoul, Republic of Korea, Min, G.Y., Sogang University, Seoul, Republic of Korea, Park, N.Y., Sogang University, Seoul, Republic of Korea, Lee, K.W., Sogang University, Seoul, Republic of Korea, Cho, Y.J., Seoul National University, Seoul, Republic of Korea, Chun, J.S., Seoul National University, Seoul, Republic of Korea, Kim, K.S., Sogang University, Seoul, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Seoul Korean Society for Applied Microbiology 01.12.2013; 한국미생물·생명공학회
• Subjects: Biological and medical sciences; BIOREACTEUR; BIOREACTORS; BIORREACTORES; Biotechnology; Dipeptides - metabolism; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling; Gene Expression Regulation, Bacterial - drug effects; High-Throughput Nucleotide Sequencing; Peptides, Cyclic - metabolism; transcriptome,next-generation sequencing,cyclo(Phe-Pro); VIBRIO VULNIFICUS; Vibrio vulnificus - drug effects; Vibrio vulnificus - genetics; 생물학; Gene; Phenylalanine; Transcriptomics; next-generation sequencing; Transcriptome; Vibrio vulnificus; Biofilm; Proline; Bacteria; Vibrionaceae; Sequencing; cyclo(Phe-Pro)
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.1308.08068

Diketopiperazine is produced by various organisms, including bacteria, fungi, and animals, and has been suggested as a novel signal molecule involved in the modulation of genes with various biological functions. Vibrio vulnificus, which causes septicemia in humans, produces cyclo(L-phenylalanine-L-proline) (cFP). To understand the biological roles of cFP, the effect of the compound on the expression of the total mRNA in V. vulnificus was assessed by nextgeneration sequencing. Based on the transcriptomic analysis, we classified the cFP-regulated genes into functional categories and clustered them according to the expression patterns resulted from treatment with cFP. From a total of 4,673 genes, excepting the genes encoding tRNA in V. vulnificus, 356 genes were up-regulated and 602 genes were down-regulated with an RPKM (reads per kilobase per million) value above 3. The genes most highly induced by cFP comprised those associated with the transport and metabolism of inorganic molecules, particularly iron. The genes negatively regulated by cFP included those associated with energy production and conversion, as well as carbohydrate metabolism. Noticeably, numerous genes related with biofilm formation were modulated by cFP. We demonstrated that cFP interferes significantly with the biofilm formation of V. vulnificus.