Performance of two isolates of Isaria fumosorosea from hot climate zones in solid and submerged cultures and thermotolerance of their propagules

• Published in: World journal of microbiology & biotechnology Vol. 29; no. 2; pp. 309 - 317
• Main Authors: Esther, Carrillo-Pérez, Erika, Acosta-Smith, Rosa María, Montesinos-Cisneros, de la Torre, Mayra
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.02.2013; Springer Nature B.V
• Subjects: Agriculture; Analysis; Animals; Anniversaries; Applied Microbiology; Batch Cell Culture Techniques; Biochemistry; Biomedical and Life Sciences; Biotechnology; Climate; Environmental Engineering/Biotechnology; Enzymes; Fungal infections; Fungi; Galleria mellonella; Hot Temperature; Hypocreales - chemistry; Hypocreales - growth & development; Hypocreales - isolation & purification; Larva - growth & development; Larva - microbiology; Larvae; Lepidoptera - microbiology; Life Sciences; Mexico; Microbiology; Moths - growth & development; Moths - microbiology; Original Paper; Pathogens; Pest Control, Biological - methods; Pesticides; Planococcus Insect - microbiology; Precipitation; Rice; Sea level; Spores, Fungal - chemistry; Spores, Fungal - growth & development; Spores, Fungal - isolation & purification; Studies; Temperature; Tropical environments; Mexico; Pathogenicity; Thermotolerance; Submerged culture; Solid culture
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-012-1184-1

Isaria fumosorosea frequently causes mycosis of agricultural pests in the hot semiarid and dry tropical regions of Mexico. Because temperature tolerance restricts the use of fungal biopesticides, we investigated two isolates from these areas for possible development into mycoinsecticides for use in hot weather agricultural zones. We studied the effects of culture system (solid or submerged cultures) and temperature on the fungal growth, extracellular enzyme production, pathogenicity, and thermotolerance of the produced propagules. Between 20 and 28 °C, the specific growth rates of the isolate PCC were higher on solid media, but in the submerged culture, the isolate P43A grew faster even at temperatures of up to 34 °C. On solid media, P43A produced 1.5-fold more proteases than PCC, but in the submerged culture, both strains had similar activities. Under the same culture conditions, PCC produced a blastospore:conidia ratio of 1:2, and P43A produced a ratio of 1:5. PCC aerial conidia had the shortest Lethal Time 50 (LT 50 , the time to reach 50 % mortality) against Galleria mellonella larvae, but LT 50 was equal for the aerial conidia and the submerged propagules of P43A and PCC. The submerged and aerial propagules of P43A were more thermotolerant than those of PCC. Each isolate performed differently in each culture system, and we concluded that the intended production method should be included as a criterion for screening of entomopathogenic fungus. We found that thermotolerance is a specific characteristic of an isolate from a given species. Because of its specific characteristics, P43A shows more promise for the development of a submerged conidia-based mycoinsecticide for foliar application in aqueous form in hot climate regions.