Esterification activity and stability of Candida rugosa lipase immobilized into chitosan

• Published in: Applied biochemistry and biotechnology Vol. 98; no. 1-9; pp. 977 - 986
• Main Authors: Pereira, E.B, Castro, H.F. de, Moraes, F.F. de, Zanin, G.M
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Heidelberg Springer 2002; Springer Nature B.V
• Subjects: Acetic acid; Acids; Alcohol; Alcohols; Alcohols - chemistry; Alcohols - metabolism; Aliphatic alcohols; Beads; Bioconversions. Hemisynthesis; Biological and medical sciences; Biotechnology; Butanol; Butyric acid; Candida - enzymology; Candida rugosa; Catalysts; Chitin - analogs & derivatives; Chitosan; Design of experiments; Enzyme Stability; Enzymes, Immobilized - chemistry; Enzymes, Immobilized - metabolism; Esterification; Esters - metabolism; Ethanol; Experimental design; Factorial design; Fundamental and applied biological sciences. Psychology; half life; Hydrolysis; Kinetics; Lipase; Lipase - chemistry; Lipase - metabolism; Methods. Procedures. Technologies; Microorganisms; Organic acids; Selectivity; temperature; Stability; Enzyme; Immobilization; Triacylglycerol lipase; Esterases; Esterification; Carboxylic ester hydrolases; Fungi; Hydrolases; Fungi Imperfecti; Chitosan; Immobilized enzyme; Candida rugosa; Thallophyta; Enzymatic reaction
• ISSN: 0273-2289; 1559-0291; 0273-2289
• DOI: 10.1385/ABAB:98-100:1-9:977

Microbial lipase from Candida rugosa immobilized into porous chitosan beads was tested for esterification selectivity with butanol and different organic acids (C2-C12), and butyric acid and different aliphatic alcohols (C2-C10). After 24 h, the acids tested achieved conversions of about 40-45%. Acetic acid was the only exception, and in this case butanol was not consumed. Different alcohols led to butyric acid conversions >40%, except for ethanol, in which case butyric acid was converted only 26%. The system's butanol and butyric acid were selected for a detailed study by employing an experimental design. The influence of temperature, initial catalyst concentration, and acid:alcohol molar ratio on the formation of butyl butyrate was simultaneously investigated, employing a 2(3) full factorial design. The range studied was 37-50 degrees C for temperature (X1), 1.25-2.5% (w/v) for the catalyst concentration (X2), and 1 and 2 for the acid:alcohol molar ratio (X3). Catalyst concentration (X2) was found to be the most significant factor and its influence was positive. Maximum ester yield (83%) could be obtained when working at the lowest level for temperature (37 degrees C), highest level for lipase concentration (2.5% [w/v]), and center level of acid:alcohol molar ratio (1.5). The immobilized lipase was also used repeatedly in batch esterification reactions of butanol with butyric acid, revealing a half-life of 86 h.