Construction and Generation of a Recombinant Senecavirus a Stably Expressing the NanoLuc Luciferase for Quantitative Antiviral Assay

Senecavirus A (SVA), also known as Seneca Valley virus, is a recently emerged picornavirus that can cause swine vesicular disease, posing a great threat to the global swine industry. A recombinant reporter virus (rSVA-Nluc) stably expressing the nanoluciferase (Nluc) gene between SVA 2A and 2B was d...

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Published inFrontiers in microbiology Vol. 12; p. 745502
Main Authors Guo, Xiaoran, Zhao, Kuan, Liu, Xiaona, Lei, Baishi, Zhang, Wuchao, Li, Xiuli, Yuan, Wanzhe
Format Journal Article
LanguageEnglish
Published Frontiers Media S.A 01.10.2021
Subjects
antiviral screening
Microbiology
nanoluciferase
neutralization test
Senecavirus A (SVA)
siRNA
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Summary:Senecavirus A (SVA), also known as Seneca Valley virus, is a recently emerged picornavirus that can cause swine vesicular disease, posing a great threat to the global swine industry. A recombinant reporter virus (rSVA-Nluc) stably expressing the nanoluciferase (Nluc) gene between SVA 2A and 2B was developed to rapidly detect anti-SVA neutralizing antibodies and establish a high-throughput screen for antiviral agents. This recombinant virus displayed similar growth kinetics as the parental virus and remained stable for more than 10 passages in BHK-21 cells. As a proof-of-concept for its utility for rapid antiviral screening, this reporter virus was used to rapidly quantify anti-SVA neutralizing antibodies in 13 swine sera samples and screen for antiviral agents, including interferons ribavirin and interferon-stimulated genes (ISGs). Subsequently, interfering RNAs targeting different regions of the SVA genome were screened using the reporter virus. This reporter virus (rSVA-Nluc) represents a useful tool for rapid and quantitative screening and evaluation of antivirals against SVA.
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Reviewed by: Qinfeng Huang, University of Minnesota Twin Cities, United States; Rui Guo, Brigham and Women’s Hospital and Harvard Medical School, United States
Edited by: Chunfu Zheng, University of Calgary, Canada
This article was submitted to Virology, a section of the journal Frontiers in Microbiology
These authors have contributed equally to this work and share first authorship
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2021.745502