Pre-endurance training prevents acute alcoholic liver injury in rats through the regulation of damaged mitochondria accumulation and mitophagy balance

• Published in: Hepatology international Vol. 8; no. 3; pp. 425 - 435
• Main Authors: Ma, Guo-dong, Liu, Yan-huan, Zhang, Qing-lai, Zhang, Bao-guo, Zhao, Ning, Wang, Qiu-ling, Wang, Xiao-di
• Format: Journal Article
• Language: English
• Published: India Springer India 01.07.2014; Springer Nature B.V
• Subjects: Colorectal Surgery; Hepatology; Medicine; Medicine & Public Health; Original Article; Surgery; Alcohol; Endurance training; Mitophagy; Acute hepatic injury
• ISSN: 1936-0533; 1936-0541
• DOI: 10.1007/s12072-014-9529-5

Aim The aim of this study is to investigate the effect of pre-endurance training on the prevention of alcohol-induced acute hepatic injury and on hepatic mitophagy. Methods Forty-eight male Sprague–Dawley rats were randomly divided into four groups: (1) control group, (2) 12-week exercise training group, (3) 5-day alcohol intake group, and (4) 12-week exercise training plus 5-day alcohol intake group. The rats were examined to determine the following: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3), hypoxia-inducible factor-1α (HIF-1α), cytochrome P450 2E1 (CYP2E1), alcohol dehydrogenase (ADH), microtubule-associated protein 1 light chain 3 (LC3II), Beclin1 mRNA and protein expressions, mitochondrial reactive oxygen species (ROS) production, mitochondrial thiobarbituric acid-reactive substances (TBARS) level, aconitase and ATP synthase activities, mitochondrial inner membrane potential, NADH/NAD + ratio, triglyceride (TG), the number of mtDNA and mitochondrial respiration functions in liver tissue, and serum ALT and AST. Results Pre-endurance training attenuated acute alcohol treatment-induced increase in mitochondrial TBARS, ROS production, NADH/NAD + ratio, state 4 respiration rate, TG, serum ALT and AST, as well as BNIP3, HIF-1α, LC3II, and Beclin 1 mRNA and protein levels, however, CYP2E1 and ADH mRNA and protein levels unchanged. Meanwhile, it attenuated the acute alcohol intake-induced decrease in aconitase activity, inner mitochondrial membrane potential (Δ ψ ), ATP synthase activity, state 3 respiration rate, respiratory control ratio, and the number of mtDNA. Conclusion Pre-endurance training can decrease acute alcohol intake-induced damaged mitochondria accumulation and reduced acute alcohol intake-induced mitophagy, which built a new balance between mitophagy and damaged mitochondria accumulation.