Glucose and cationic amino acid transporter expression in growing chickens ( Gallus gallus domesticus)

• Published in: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology Vol. 138; no. 4; pp. 515 - 525
• Main Authors: Humphrey, Brooke D., Stephensen, Charles B., Calvert, Chris C., Klasing, Kirk C.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.08.2004
• Subjects: Aging - genetics; Amino Acid Transport Systems, Basic - genetics; amino acid transporters; animal tissues; Animals; Arginine; broiler chickens; CAT-1; CAT-2; CAT-3; Chickens - genetics; Chickens - growth & development; chicks; gene expression; Gene Expression Regulation, Developmental; Glucose; glucose transporters; GLUT-1; GLUT-2; GLUT-3; Growth; Lymphocyte; Lysine; Male; messenger RNA; Monosaccharide Transport Proteins - genetics; Ontogeny; Protein Isoforms - genetics; RNA, Messenger - genetics; RNA, Messenger - metabolism; Time Factors; tissue distribution; Transport; Gastroc; GLUT; Growth; Ontogeny; Glucose; Pec; Arginine; LOW; Lysine; nd; CAT; Transport; Lymphocyte
• ISSN: 1095-6433; 1531-4332
• DOI: 10.1016/j.cbpb.2004.06.016

Tissue glucose transporter (GLUT1–3) and cationic amino acid transporter (CAT1–3) mRNA expression was determined in growing broiler chicks posthatch. In two experiments, tissues were either collected on days 1, 3 and 7 or days 1 and 14 posthatch. Heart and liver were the only tissues expressing a GLUT isoform on day 1. All tissues expressed a GLUT isoform on day 7 except for the thymus. Most tissues expressing a CAT isoform on day 1 decreased mRNA levels through day 7 ( P<0.05), except for bursa CAT-1 which tended to increase ( P=0.05). The thymus and spleen did not express any CAT isoform mRNA until day 7. The liver was the only tissue expressing GLUT-2 mRNA through day 14. On day 14, GLUT-1, CAT-1 and CAT-2 mRNA were differentially expressed across tissues ( P<0.05). High-affinity GLUT and CAT mRNA expression was highest in the heart and bursa, respectively ( P<0.05). Total CAT mRNA expression was greatest in the bursa ( P<0.05). The thymus had the lowest high affinity GLUT and total CAT mRNA expression on day 14 posthatch. Therefore, T lymphocytes within the thymus may be most susceptible to glucose and cationic amino acid supply.