Reactivity of MEST-1 (antigalactofuranose) with Trypanosoma cruzi glycosylinositol phosphorylceramides (GIPCs): immunolocalization of GIPCs in acidic vesicles of epimastigotes

Using confocal microscopy, MEST-1-positive immunofluorescence was observed within various Trypanosoma cruzi forms, except in cell-derived trypomastigotes. Glycosylinositol phosphorylceramides were identified by thin-layer chromatography immunostaining as the antigens recognized by MEST-1 in these pa...

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Published inClinical and diagnostic laboratory immunology Vol. 8; no. 5; pp. 1031 - 1035
Main Authors Suzuki, E, Mortara, R A, Takahashi, H K, Straus, A H
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.09.2001
SeriesNote
Subjects
Animals
Antibodies, Monoclonal - metabolism
Antibodies, Protozoan - metabolism
Antigens, Protozoan - immunology
Antigens, Protozoan - metabolism
Furans - immunology
Galactose - immunology
Glycosphingolipids - immunology
Glycosphingolipids - metabolism
glycosylinositol phosphorylceramides
Hydrogen-Ion Concentration
Mice
Microbial Immunology
Transport Vesicles - immunology
Transport Vesicles - metabolism
Trypanosoma cruzi
Trypanosoma cruzi - growth & development
Trypanosoma cruzi - immunology
Trypanosoma cruzi - metabolism
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Summary:Using confocal microscopy, MEST-1-positive immunofluorescence was observed within various Trypanosoma cruzi forms, except in cell-derived trypomastigotes. Glycosylinositol phosphorylceramides were identified by thin-layer chromatography immunostaining as the antigens recognized by MEST-1 in these parasites. In epimastigotes, labeling of MEST-1 coincided with acidic vesicles, indicating an internal localization of these glycoconjugates.
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Corresponding author. Mailing address: Departamento de Bioquímica, Universidade Federal de São Paulo, Escola Paulista de Medicina, Rua Botucatu 862, São Paulo, SP 04023-900, Brazil. Phone and fax: 55-11-5579-2509. E-mail: straus.bioq@epm.br.
ISSN:1071-412X
1098-6588
DOI:10.1128/CDLI.8.5.1031-1035.2001