Tumor-Infiltrated CD8+ T Cell 10-Gene Signature Related to Clear Cell Renal Cell Carcinoma Prognosis

• Published in: Frontiers in immunology Vol. 13; p. 930921
• Main Authors: Wang, Jie, Huang, Feifan, Zhao, Jingjie, Huang, Peng, Tan, Junhua, Huang, Meiying, Ma, Ruiying, Xiao, Yu, He, Siyuan, Wang, Zechen, Shen, Jiajia, Lu, Heming, Meng, Lingzhang
• Format: Journal Article
• Language: English
• Published: Frontiers Media S.A 24.06.2022
• Subjects: CD8+ T cell; clear cell renal cell carcinoma; Immunology; neutrophil metabolism; papillary renal cell carcinoma; scRNA-seq
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2022.930921

Clear cell renal cell carcinoma (ccRCC) usually affects multiple organs (e.g., bone and brain), and patient prognosis is usually poor. Although it is known that CD8+ T cell infiltration can potentially alleviate ccRCC progression, few studies have concentrated on the correlation between CD8+ T cell infiltration and ccRCC prognosis. In this study, ten genes expressed by infiltrated CD8+ T cells (i.e., AMD1, CCSER2, CIB1, DRAP1, HMGB2, HMGN1, NPIPB5, PTP4A2, RORA , and SAP18 ) were suggested as potential ccRCC prognostic biomarkers, by using next-generation sequencing (i.e. bulk sequencing and single-cell sequencing) of ccRCC, papillary renal cell carcinoma (papRCC), and control kidney biopsies. Specifically, we identified four genes (i.e., CCSER2, DRAP1, NPIPB5 , and SAP18 ) as potential novel prognostic biomarkers for ccRCC. It is noteworthy that SAP18 derived from CD8+ T cells negatively correlates to Atg7+ neutrophils in ccRCC, compared with papRCC, indicating a potential decreased neutrophil metabolic function in autophagy and fatty acids. This study elucidated the protective role of infiltrated CD8+ T cells in ccRCC and identified ten candidate genes related to an improved prognosis in patients with ccRCC.