Cell response to collagen-calcium phosphate cement scaffolds investigated for nonviral gene delivery

• Published in: Journal of materials science. Materials in medicine Vol. 22; no. 4; pp. 887 - 897
• Main Authors: Perez, R. A., Ginebra, M. P., Spector, M.
• Format: Journal Article Publication
• Language: English
• Published: Boston Springer US 01.04.2011; Springer; Springer Nature B.V
• Subjects: Alkaline Phosphatase - chemistry; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Animals; Applied cell therapy and gene therapy; Biological and medical sciences; Biomaterials; Biomedical Engineering and Bioengineering; Biomedical materials; Bone Cements - chemistry; Bone Morphogenetic Protein 7 - metabolism; Calcium; Calcium Phosphates - chemistry; Cell Proliferation; Cells, Cultured; Cellular biology; Ceramics; Chemistry and Materials Science; Ciència dels materials; Collagen; Collagen - chemistry; Composites; Enginyeria dels materials; Gene expression; Gene Transfer Techniques; Glass; Goats; Materials Science; Medical sciences; Mesenchymal Stromal Cells - cytology; Models, Chemical; Natural Materials; Orthopedic surgery; Plasmids - metabolism; Polymer Sciences; Regenerative Medicine/Tissue Engineering; Surfaces and Interfaces; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Swine; Technology. Biomaterials. Equipments; Thin Films; Transfusions. Complications. Transfusion reactions. Cell and gene therapy; X-Ray Diffraction; Àrees temàtiques de la UPC; Collagen Scaffold; Gene Delivery Vehicle; Scaffold Group; Calcium Phosphate Cement; Protein Bone Morphogenetic Protein; Treatment; Collagen; Glycoprotein; Animal protein; Calcium phosphate; Biomaterial; Scaffold; Gene therapy; Biomedical engineering; Genetic transfer
• ISSN: 0957-4530; 1573-4838
• DOI: 10.1007/s10856-011-4308-5

Collagen-hydroxyapatite (HA) scaffolds for the non-viral delivery of a plasmid encoding the osteoinductive protein bone morphogenetic protein (BMP)-7 were developed. The collagen-HA was obtained by the combination of calcium phosphate cement in a collagen template. The effect on cell behavior of increasing amounts of HA in the scaffolds was evaluated. Collagen-HA scaffolds containing 13, 23 or 83 wt% HA were prepared. Cell proliferation was reduced in the 83% HA scaffold after 1 day compared to 13 and 23% HA, but by 14 days the number of cells in 83% HA considerably increased. Alkaline phosphatase (ALP) activity was 8 times higher for the 83% HA scaffolds. BMP-7 plasmid was incorporated into the 83% HA scaffold. The transfection was low, although significant levels of BMP7 were expressed, associated with an increase in cell proliferation.