Effect of Chia oil ( Salvia Hispanica) rich in ω -3 fatty acids on the eicosanoid release, apoptosis and T -lymphocyte tumor infiltration in a murine mammary gland adenocarcinoma

• Published in: Prostaglandins, leukotrienes and essential fatty acids Vol. 77; no. 1; pp. 21 - 28
• Main Authors: Espada, C.E, Berra, M.A, Martinez, M.J, Eynard, A.R, Pasqualini, M.E
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.07.2007; Elsevier
• Subjects: 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid - metabolism; Adenocarcinoma - drug therapy; Adenocarcinoma - immunology; Adenocarcinoma - metabolism; Advanced Basic Science; Animals; Apoptosis; Biological and medical sciences; Cell Membrane - metabolism; Eicosanoids - metabolism; Endocrinology & Metabolism; Fatty Acids, Omega-3 - analysis; Fatty Acids, Unsaturated - metabolism; Female; Fundamental and applied biological sciences. Psychology; Gynecology. Andrology. Obstetrics; Lymphocytes, Tumor-Infiltrating - drug effects; Male; Mammary gland diseases; Mammary Neoplasms, Experimental - drug therapy; Mammary Neoplasms, Experimental - immunology; Mammary Neoplasms, Experimental - metabolism; Medical sciences; Mice; Mice, Inbred BALB C; Mitosis; Phytotherapy; Plant Oils - chemistry; Plant Oils - therapeutic use; Prostaglandin-Endoperoxide Synthases - metabolism; Salvia - chemistry; T-Lymphocytes - immunology; Tumors; Vertebrates: endocrinology; Breast adenocarcinoma; Rodentia; Polyunsaturated fatty acid; Lipids; Malignant tumor; n-3 fatty acid; Mammary gland diseases; Vertebrata; Mammalia; Mouse; Cell death; Animal; Eicosanoid; T-Lymphocyte; Unsaturated fatty acid; Infiltration; Mammary gland; Release; Apoptosis
• ISSN: 0952-3278; 1532-2823
• DOI: 10.1016/j.plefa.2007.05.005

Abstract We investigated the effects of certain dietary polyunsaturated fatty acids (PUFAs) and related eicosanoids on the growth and metastasis formation of a murine mammary gland adenocarcinoma. Salvia hispanica (ChO) and Carthamus tinctorius (SaO) vegetable oil sources of ω -3 and -6 PUFAs and a commercial diet as control (CO), were used. We analysed fatty acids of neoplastic cells (NC) membranes by GLC; the eicosanoids 12- HETE and 12-HHT (LOX and COX metabolites) by HPLC and apoptosis and T -lymphocyte infiltration by flow cytometry and microscopy. NC from ChO groups showed lower levels of arachidonic acid and of both eicosanoids compared to SaO and CO ( p <0.05). The ChO diet decreased the tumor weight and metastasis number ( p <0.05). Apoptosis and T -lymphocyte infiltration were higher and mitosis decreased with respect to the other diets ( p <0.05). Present data showed that ChO, an ancient and almost unknown source of ω -3, inhibits growth and metastasis in this tumor model.