Nanorings from Concatemeric DNA: Chemical Modification Drives Nanostructure Formation

Self-assembly of DNA concatemers from native duplexes and those containing non-nucleotidic bridges of varying polarity composed of repeating oligo(ethylene glycol) phosphates -O(CH2CH2O)(n)PO2- or α,Ω-alkanediol phosphates -O(CH2)10OPO2(-)- units was compared. The structures obtained were characteri...

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Bibliographic Details
Published inJournal of nanoscience and nanotechnology Vol. 15; no. 6; p. 4170
Main Authors Vinogradova, Olga A, Lomzov, Alexander A, Shevelev, Georgiy Yu, Sheglov, Dmitrii V, Latyshev, Alexander V, Stetsenko, Dmitry A, Pyshnyi, Dmitrii V
Format Journal Article
LanguageEnglish
Published United States 01.06.2015
Subjects
Base Sequence
Deoxyribonucleases - metabolism
DNA, Concatenated - chemistry
DNA, Concatenated - metabolism
Ethylene Glycol - chemistry
Microscopy, Atomic Force
Molecular Sequence Data
Nanostructures - chemistry
Oligonucleotides
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Summary:Self-assembly of DNA concatemers from native duplexes and those containing non-nucleotidic bridges of varying polarity composed of repeating oligo(ethylene glycol) phosphates -O(CH2CH2O)(n)PO2- or α,Ω-alkanediol phosphates -O(CH2)10OPO2(-)- units was compared. The structures obtained were characterised by polyacrylamide gel electrophoresis, enzymatic digestion and AFM. Our results have revealed that chemically-modified duplexes favour self-termination of concatemer growth and yield up to 35% of nanosized DNA rings.
ISSN:1533-4880
DOI:10.1166/jnn.2015.9796