A Novel Antimicrobial Peptides From Pine Needles of Pinus densiflora Sieb. et Zucc. Against Foodborne Bacteria

Pine needles are used in several East Asian countries as food or traditional medicine. It contains functional components that exhibit a wide spectrum of pharmacological effects such as antioxidant, antimicrobial, anti-diabetic, and anti-inflammatory activities. We determined and characterized the no...

Full description

Saved in:
Bibliographic Details
Published inFrontiers in microbiology Vol. 12; p. 662462
Main Authors Lee, Junho, Kang, Hee Kyoung, Cheong, Hyeonsook, Park, Yoonkyung
Format Journal Article
LanguageEnglish
Published Frontiers Media S.A 20.05.2021
Subjects
antimicrobial peptide
foodborne bacteria
Microbiology
pine needles
Pinus densiflora
ultrafiltration
Online AccessGet full text

Cover

More Information
Summary:Pine needles are used in several East Asian countries as food or traditional medicine. It contains functional components that exhibit a wide spectrum of pharmacological effects such as antioxidant, antimicrobial, anti-diabetic, and anti-inflammatory activities. We determined and characterized the novel antimicrobial peptides (AMPs) isolated from Pinus densiflora Sieb. et Zucc. The four active pine-needle (PN) peptides showed antimicrobial activity against foodborne bacteria with minimum inhibitory concentration (MIC) values within the range of 8–128 μg/ml. PN peptides showed no detectable hemolytic activity or cytotoxicity at the antimicrobial concentrations. The N -terminal amino acid sequence of the PN5 was identified using Edman degradation and Antimicrobial Peptide Database (APD) homology analysis showed that it was not identical to any other plant peptide. This suggests that PN5 can serve as an alternative therapeutic agent to be used in the food industry.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Edited by: Octavio Luiz Franco, Catholic University of Brasilia (UCB), Brazil
Reviewed by: Eugene A. Rogozhin, Institute of Bioorganic Chemistry, Russian Academy of Sciences (RAS), Russia; Alba Pérez-Cataluña, Institute of Agrochemistry and Food Technology (IATA), Spain
These authors have contributed equally to this work and share first authorship
This article was submitted to Antimicrobials, Resistance and Chemotherapy, a section of the journal Frontiers in Microbiology
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2021.662462