Molecularly Imprinted Electrochemical Sensor-Based Fe2O3@MWCNTs for Ivabradine Drug Determination in Pharmaceutical Formulation, Serum, and Urine Samples
Ivabradine hydrochloride (IVR) is a medically important drug because of its ability to lower the heart rate. Techniques reported for IVR determination were expensive, laborious, besides being of poor selectivity. In this study, iron oxide @ carbon nanotube (Fe 2 O 3 @MWCNTs) nanocomposite and molecu...
Saved in:
Published in | Frontiers in bioengineering and biotechnology Vol. 9; p. 648704 |
---|---|
Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
08.04.2021
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Ivabradine hydrochloride (IVR) is a medically important drug because of its ability to lower the heart rate. Techniques reported for IVR determination were expensive, laborious, besides being of poor selectivity. In this study, iron oxide @ carbon nanotube (Fe
2
O
3
@MWCNTs) nanocomposite and molecularly imprinted polymer (MIP) were synthesized and used in the fabrication of carbon paste electrodes (CPEs) for the potentiometric detection of IVR in biological and pharmaceutical samples. CPEs of the best sensor were formulated from graphite (41 wt%) as a carbon source, MIP (3 wt.%) as an ionophore, Fe
2
O
3
@MWCNTs (5 wt%) as a modifier, and nitrophenyl octyl ether (NPOE, 51 wt.%) as a conductive oil so-called plasticizer. The best sensor exhibits a Nernstian slope (response) of 56 mV decade
–1
within the IVR concentration range from 1.0 × 10
–3
M to 9.8 × 10
–8
M with high selectivity against interfering species (ascorbic, maltose, glucose, lactose, dopamine, glycine) over those reported earlier. The use of Fe
2
O
3
@MWCNTs together with MIP in the electrode formulation was found to improve the limit of detection (LOD) from 630 to 98 nM along with high reversibility, a short response time of 30 s, and a good lifetime of more than 2 weeks. The sandwich membrane (SMM) method was used to quantify the H-bonding complexing strength of the MIP binding sites for IVR with Log β
ILn
= 11.33. The constructed sensors were successfully applied for the IVR determination in blood serum, urine, and commercial formulations (Savapran
®
) with high sensitivity. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Pramod K. Kalambate, Chulalongkorn University, Thailand; Omer Sadak, Ardahan University, Turkey; Anu Prathap, PEC University of Technology, India Edited by: Anandhakumar Sundaramurthy, SRM Institute of Science and Technology, India This article was submitted to Nanobiotechnology, a section of the journal Frontiers in Bioengineering and Biotechnology |
ISSN: | 2296-4185 2296-4185 |
DOI: | 10.3389/fbioe.2021.648704 |