Comparative genomic analysis reveals cellulase plays an important role in the pathogenicity of Setosphaeria turcica f. sp. zeae
Setosphaeria turcica f. sp. zeae and S. turcica f. sp. sorghi , the two formae speciales of S. turcica , cause northern leaf blight disease of corn and sorghum, respectively, and often cause serious economic losses. They have obvious physiological differentiation and show complete host specificity....
Saved in:
Published in | Frontiers in microbiology Vol. 13; p. 925355 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
22.07.2022
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Setosphaeria turcica
f. sp.
zeae
and
S. turcica
f. sp.
sorghi
, the two formae speciales of
S. turcica
, cause northern leaf blight disease of corn and sorghum, respectively, and often cause serious economic losses. They have obvious physiological differentiation and show complete host specificity. Host specificity is often closely related to pathogen virulence factors, including secreted protein effectors and secondary metabolites. Genomic sequencing can provide more information for understanding the virulence mechanisms of pathogens. However, the complete genomic sequence of
S. turcica
f. sp.
sorghi
has not yet been reported, and no comparative genomic information is available for the two formae speciales. In this study,
S. turcica
f. sp.
zeae
was predicted to have fewer secreted proteins, pathogen-host interaction (PHI) genes and carbohydrate-active enzymes (CAZys) than
S. turcica
f. sp.
sorghi
. Fifteen and 20 polyketide synthase (PKS) genes were identified in
S. turcica
f. sp.
zeae
and
S. turcica
f. sp.
sorghi
, respectively, which maintained high homology. There were eight functionally annotated effector protein-encoding genes specifically in
S. turcica
f. sp.
zeae
, among which the encoding gene
StCEL2
of endo-1, 4-β-D-glucanase, an important component of cellulase, was significantly up-regulated during the interaction process. Finally, gluconolactone inhibited cellulase activity and decreased infection rate and pathogenicity, which indicates that cellulase is essential for maintaining virulence. These findings demonstrate that cellulase plays an important role in the pathogenicity of
S. turcica
f. sp.
zeae
. Our results also provide a theoretical basis for future research on the molecular mechanisms underlying the pathogenicity of the two formae speciales and for identifying any associated genes. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Boqiang Li, Institute of Botany (CAS), China Reviewed by: Yuli Dai, Fujian Academy of Agricultural Sciences, China; Wenxing Liang, Qingdao Agricultural University, China; Roshni R. Kharadi, Michigan State University, United States This article was submitted to Microbe and Virus Interactions With Plants, a section of the journal Frontiers in Microbiology |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2022.925355 |