Amentoflavone Induces Cell-cycle Arrest, Apoptosis, and Invasion Inhibition in Non-small Cell Lung Cancer Cells

• Published in: Anticancer research Vol. 41; no. 3; pp. 1357 - 1364
• Main Authors: Chen, Wei-Ting, Chen, Cheng-Hsien, Su, Hung-Tai, Yueh, Po-Fu, Hsu, Fei-Ting, Chiang, I-Tsang
• Format: Journal Article
• Language: English
• Published: Greece International Institute of Anticancer Research 01.03.2021
• Subjects: Apoptosis; Apoptosis - drug effects; Assaying; Biflavonoids - pharmacology; Carcinoma, Non-Small-Cell Lung - metabolism; Carcinoma, Non-Small-Cell Lung - pathology; Caspase-3; Cell Cycle Checkpoints - drug effects; Cell Line, Tumor; Cell Movement - drug effects; Cell Survival - drug effects; Cell viability; Chemotherapy; Cytochrome P-450 CYP2C9 Inhibitors - pharmacology; Flow cytometry; G1 Phase Cell Cycle Checkpoints; Growth factors; Growth inhibition; Herbal medicine; Humans; Immunofluorescence; Kinases; Lung cancer; Lung Neoplasms - metabolism; Lung Neoplasms - pathology; Matrix metalloproteinase; Matrix metalloproteinases; Matrix Metalloproteinases - metabolism; Medicinal plants; Metalloproteinase; Neoplasm Invasiveness; NF-kappa B - metabolism; NF-κB protein; Non-small cell lung carcinoma; Reporter gene; Signal Transduction - drug effects; Small cell lung carcinoma; Vascular endothelial growth factor; Vascular Endothelial Growth Factor A - metabolism; Amentoflavone; non-small cell lung cancer; apoptosis; nuclear factor-kappa B; invasion
• ISSN: 0250-7005; 1791-7530
• DOI: 10.21873/anticanres.14893

Amentoflavone, an effective compound derived from medicinal plants, has been shown to boost therapeutic efficacy of chemotherapy in non-small cell lung cancer (NSCLC). However, anti-NSCLC effect of amentoflavone is ambiguous. The major purpose of the present study was to verify the inhibitory effects of amentoflavone in NSCLC cells. The effects of amentoflavone on growth and invasion of NSCLC CL-1-5-F4 cells were evaluated by cell viability assay, flow cytometry, colony formation assay, nuclear factor-kappa B (NF-κB) reporter gene assay, immunofluorescence staining, transwell invasion, and western blot assay. Amentoflavone effectively induced cell growth inhibition, G1 cell-cycle arrest, apoptosis, and suppression of invasion. Furthermore, amentoflavone not only triggered expression of p27, cleaved caspase-3, -8 also reduced NF-κB signaling, protein levels of matrix metalloproteinase (MMP)-2, -9, Cyclin-D1, and vascular endothelial growth factor (VEGF). Cell-cycle arrest, apoptosis induction, NF-κB signaling inhibition are associated with amentoflavone-inhibited growth and invasion of NSCLC cells.