Significant Gene Biomarker Tyrosine Kinase Non-receptor 2 Mediated Cell Proliferation and Invasion in Colon Cancer

Objective: This study aimed to investigate the expression and biological functions of TNK2 and miR-125a-3p in colon cancer. Materials and methods: The expression of TNK2 and miR-125a-3p in colon cancer tissues was analyzed using data deposited on public databases including UALCAN and ONCOMINE. We ve...

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Published inFrontiers in genetics Vol. 12; p. 653657
Main Authors Ling, Sunkai, He, Yanru, Li, Xiaoxue, Ma, Yu, Li, Yuan, Kong, Bo, Huang, Peilin
Format Journal Article
LanguageEnglish
Published Frontiers Media S.A 04.08.2021
Subjects
bioinformaticanalysis
biomarker
colon cancer
Genetics
miR-125a-3p
TNK2
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Summary:Objective: This study aimed to investigate the expression and biological functions of TNK2 and miR-125a-3p in colon cancer. Materials and methods: The expression of TNK2 and miR-125a-3p in colon cancer tissues was analyzed using data deposited on public databases including UALCAN and ONCOMINE. We verified their expression in colon cancer cell lines by RT-qPCR and western blotting. By regulating the expression of TNK2 and miR-125a-3p in colon cancer cells, their functions and potential mechanisms were explored. Results: TNK2 was overexpressed in colon cancer cell lines, and it was found to directly bind to miR-125a-3p, which was downregulated in these cell lines. Their expression affected the proliferation and invasion of colon cancer cells. Additionally, colon cancer patients with lower TNK2 expression had better prognoses than those with higher TNK2 expression. Conclusion: Our results indicated that TNK2 and miR-125a-3p play critical roles in colon cancer, and could also serve as biomarkers for the diagnosis and prognosis of this malignant disease.
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Reviewed by: Nan Lin, Regeneron Genetic Center, United States; Weifeng Ding, Nantong University, China
This article was submitted to Cancer Genetics, a section of the journal Frontiers in Genetics
Edited by: Olorunseun O. Ogunwobi, Hunter College (CUNY), United States
This author takes responsibility for all aspects of the reliability and unbiased presentation of the data interpretation
ISSN:1664-8021
1664-8021
DOI:10.3389/fgene.2021.653657