Clinical significance of miRNA-21, -103, -129, -150 in serous ovarian cancer

• Published in: Archives of gynecology and obstetrics Vol. 297; no. 3; pp. 741 - 748
• Main Authors: Wilczyński, Miłosz, Żytko, Ewelina, Danielska, Justyna, Szymańska, Bożena, Dzieniecka, Monika, Nowak, Marek, Malinowski, Jakub, Owczarek, Dariusz, Wilczyński, Jacek R.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.03.2018; Springer Nature B.V
• Subjects: Aged; Biomarkers, Tumor - metabolism; Carcinoma, Ovarian Epithelial - genetics; Carcinoma, Ovarian Epithelial - mortality; Carcinoma, Ovarian Epithelial - pathology; Case-Control Studies; Clinical significance; Cystadenocarcinoma, Serous - genetics; Cystadenocarcinoma, Serous - mortality; Cystadenocarcinoma, Serous - pathology; Endocrinology; Female; Gene Expression Regulation, Neoplastic; Gynecologic Oncology; Gynecology; Human Genetics; Humans; Medicine; Medicine & Public Health; Metastasis; MicroRNAs - genetics; MicroRNAs - metabolism; Middle Aged; Neoplasm Staging; Obstetrics/Perinatology/Midwifery; Ovarian cancer; Ovarian Neoplasms - genetics; Ovarian Neoplasms - mortality; Ovarian Neoplasms - pathology; Prognosis; Real-Time Polymerase Chain Reaction; Survival Rate; Prognostic factor; miRNA; Survival; Ovarian cancer
• ISSN: 0932-0067; 1432-0711
• DOI: 10.1007/s00404-018-4660-5

Purpose We aimed to compare expression levels of miRNA-21, -103, -129, -150 in primary tumour tissues and its omental metastases from patients operated for advanced ovarian serous cancer. Expression levels of selected miRNAs were correlated with clinicopathological features, including chemosensitivity and survival. Methods We performed total RNA extraction from archival formalin-fixed paraffin-embedded tissue samples of primary serous ovarian cancer and omental metastases. The study included 48 patients with advanced ovarian cancer. The reference group consisted of 48 normal ovarian tissue samples. We performed cDNA synthesis, real time polymerase chain reaction and assessed relative expression of selected miRNAs. Results Samples derived from serous ovarian cancer were characterized by higher expression levels of miRNA-150 in comparison to omental metastases ( p  = 0.045). Furthermore, we observed that shorter progression free-survival was associated with lower levels of miRNA-150 in metastatic tissues. We did not find similar relationships for other miRNAs. Conclusions MiRNA-150 may potentially serve as a prognostic factor in advanced ovarian cancer. However, further studies are required to clearly confirm such hypothesis.