Transglycosylation activity of cellobiohydrolase I from Trichoderma longibrachiatum on synthetic and natural substrates

• Published in: Biochimica et biophysica acta Vol. 1073; no. 3; pp. 481 - 485
• Main Authors: Gusakov, Alexander V., Protas, Oksana V., Chernoglazov, Vladimir M., Sinitsyn, Arkady P., Kovalysheva, Galina V., Shpanchenko, Olga V., Ermolova, Olga V.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 09.04.1991; Elsevier
• Subjects: 4-Methylumbelliferyl ß- d-cellobioside; analogs & derivatives; cellobiose; Cellobiose - analogs & derivatives; Cellobiose - metabolism; cellulolytic microorganisms; cellulose; Cellulose - metabolism; Cellulose 1,4-beta-Cellobiosidase; Chromatography, High Pressure Liquid; enzyme activity; enzymology; Exact sciences and technology; Exo-cellobiohydrolase; Glucosides; Glucosides - metabolism; glycosidases; Glycoside Hydrolases; Glycoside Hydrolases - isolation & purification; Glycoside Hydrolases - metabolism; Glycosylation; Hymecromone; Hymecromone - analogs & derivatives; Hymecromone - metabolism; Hypocrea jecorina; isolation & purification; Mathematical analysis; Mathematics; metabolism; Models, Chemical; O-glycoside hydrolases; Potential theory; Sciences and techniques of general use; T. longibrachiatum; Transglycosylation; Trichoderma; Trichoderma - enzymology; Trichoderma reesei; Trisaccharides; Trisaccharides - metabolism; 4-Methylumbelliferyl ß- d-cellobioside; Transglycosylation; Exo-cellobiohydrolase; T. longibrachiatum; CMC; HPLC; MUF; Fungi; Purification; Enzymatic activity; Enzyme; Substrate specificity; HPLC chromatography; Kinetics; Fungi Imperfecti; Thallophyta
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/0304-4165(91)90219-7

Using 4-methylumbelliferyl (MUF) ß- d-cellobioside as a substrate, the ability of cellobiohydrolase I from Trichoderma longibrachiatum to catalyze transglycosylation has been demonstrated. At substrate concentrations greater than 2 mM, the formation of MUF-tetrasaccharide was detected using HPLC. In the course of enzymatic reaction, a concentration of the transglycosylation product passed through a maximum, since at later stages of the reaction the product was further hydrolyzed. At MUF-ß- d-cellobioside concentrations of 2–10 mM, the maximum weight content of MUF-tetrasaccharide amounted to 1–4% of the total content of saccharides. In the reaction system, containing 2.5 mM MUF-ß- d-cellobioside and 10 mM MUF-ß- d-glucoside MUF-trisaccharide was formed as the main transglycosylation product. In hydrolysis of natural substrates (cellulose and cellotriose) in the presence of MUF-ß- d-glucoside a formation of MUF-trisaccharide was also observed.