Successive walnut plantations alter soil carbon quantity and quality by modifying microbial communities and enzyme activities

• Published in: Frontiers in microbiology Vol. 13; p. 953552
• Main Authors: Luan, Haoan, Liu, Yingru, Huang, Shaohui, Qiao, Wenyan, Chen, Jie, Guo, Tengfei, Zhang, Xiaojia, Guo, Suping, Zhang, Xuemei, Qi, Guohui
• Format: Journal Article
• Language: English
• Published: Frontiers Media S.A 25.07.2022
• Subjects: extracellular enzyme activities; Microbiology; quality; soil depths; soil microbial community; soil organic carbon quantity; walnut plantation ages
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2022.953552

Knowledge of the spatial–temporal variations of soil organic carbon (SOC) quantity and quality and its microbial regulation mechanisms is essential for long-term SOC sequestration in agroecosystems; nevertheless, this information is lacking in the process of walnut plantations. Here, we used the modified Walkley-Black method, phospholipid fatty acid analysis, and micro-plate enzyme technique to analyze the evolution of SOC stocks and quality/lability as well as microbial communities and enzyme activities at different soil depths in walnut plantations with a chronosequence of 0-, 7-, 14-, and 21-years in the Eastern Taihang Mountains, China. The results indicated that long-term walnut plantations (14-and 21-years) enhanced SOC stocks, improved SOC quality/lability (as indicated by the lability index), and promoted microbial growth and activities (i.e., hydrolase and oxidase activities) in the 0–40 cm soil layers. Besides, these above-mentioned SOC-and microbial-related indices (except for oxidase activities) decreased with increasing soil depths, while oxidase activities were higher in deeper soils (40–60 cm) than in other soils (0–40 cm). The partial least squares path model also revealed that walnut plantation ages and soil depths had positive and negative effects on microbial attributes (e.g., enzyme activities, fungal and bacterial communities), respectively. Meanwhile, the SOC stocks were closely related to the fungal community; meanwhile, the bacterial community affected SOC quality/liability by regulating enzyme activities. Comprehensively, long-term walnut plantations were conducive to increasing SOC stocks and quality through altering microbial communities and activities in the East Taihang Mountains in Hebei, China.