Single-cell transcriptomic analysis of the tumor ecosystem of adenoid cystic carcinoma
Adenoid cystic carcinoma (ACC) is a malignant tumor that originates from exocrine gland epithelial cells. We profiled the transcriptomes of 49,948 cells from paracarcinoma and carcinoma tissues of three patients using single-cell RNA sequencing. Three main types of the epithelial cells were identifi...
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Published in | Frontiers in oncology Vol. 12; p. 1063477 |
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Main Authors | , , , , , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
17.11.2022
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Subjects | |
Online Access | Get full text |
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Summary: | Adenoid cystic carcinoma (ACC) is a malignant tumor that originates from exocrine gland epithelial cells. We profiled the transcriptomes of 49,948 cells from paracarcinoma and carcinoma tissues of three patients using single-cell RNA sequencing. Three main types of the epithelial cells were identified into myoepithelial-like cells, intercalated duct-like cells, and duct-like cells by marker genes. And part of intercalated duct-like cells with special copy number variations which altered with MYB family gene and EN1 transcriptomes were identified as premalignant cells. Developmental pseudo-time analysis showed that the premalignant cells eventually transformed into malignant cells. Furthermore, MYB and MYBL1 were found to belong to two different gene modules and were expressed in a mutually exclusive manner. The two gene modules drove ACC progression into different directions. Our findings provide novel evidence to explain the high recurrence rate of ACC and its characteristic biological behavior. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Ester Orlandi, National Center of Oncological Hadrontherapy, Italy This article was submitted to Head and Neck Cancer, a section of the journal Frontiers in Oncology These authors have contributed equally to this work Reviewed by: Yingying Jiang, Affiliated Hospital of Weifang Medical University, China; Gennaro Gambardella, Telethon Institute of Genetics and Medicine (TIGEM), Italy |
ISSN: | 2234-943X 2234-943X |
DOI: | 10.3389/fonc.2022.1063477 |