Protective effects of resveratrol on hepatic ischemia reperfusion injury in streptozotocin-induced diabetic rats

• Published in: Molecular and cellular biochemistry Vol. 460; no. 1-2; pp. 217 - 224
• Main Authors: Aktaş, Hanife Serife, Ozel, Yahya, Ahmad, Sarfraz, Pençe, Halime Hanim, Ayaz-Adakul, Betul, Kudas, Ilyas, Tetik, Sermin, Şekerler, Turgut, Canbey-Göret, Ceren, Kabasakal, Levent, Elcioglu, Hatice Kubra
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.10.2019; Springer; Springer Nature B.V
• Subjects: Animals; Antioxidants; Biochemistry; Biomedical and Life Sciences; Blood Glucose - metabolism; Body Weight - drug effects; Cardiology; Diabetes; Diabetes mellitus; Diabetes Mellitus, Experimental - complications; Diabetes Mellitus, Experimental - drug therapy; Glutathione; Glutathione - metabolism; Inflammation; Inflammatory response; Injuries; Ischemia; Life Sciences; Liver; Liver - drug effects; Liver - pathology; Lungs; Malondialdehyde; Malondialdehyde - metabolism; Medical Biochemistry; Medical research; Medicine, Experimental; Oncology; Oxidative stress; Parameters; Pretreatment; Protective Agents - pharmacology; Protective Agents - therapeutic use; Rats, Sprague-Dawley; Reperfusion; Reperfusion Injury - complications; Reperfusion Injury - drug therapy; Resveratrol; Resveratrol - pharmacology; Resveratrol - therapeutic use; Rodents; Streptozocin; Tissues; Tumor Necrosis Factor-alpha - metabolism; Tumor necrosis factor-TNF; Tumor necrosis factor-α; Experimental rats; Inflammation; Diabetes; Ischemia reperfusion injury; Resveratrol
• ISSN: 0300-8177; 1573-4919
• DOI: 10.1007/s11010-019-03582-z

Resveratrol (RSV) is a natural polyphenolic compound having antioxidant effects. This study was designed to investigate the protective effects of resveratrol against oxidative stress in hepatic ischemia–reperfusion (I/R) injury in streptozotocin (STZ)-induced diabetic rats. STZ was injected intraperitonally (i.p.) to 18 Sprague–Dawley albino rats, which were divided into three groups, each having six rats. First group was non-treated diabetic group (D), second diabetic group was subjected to 30 min of hepatic ischemia followed by a 45-min reperfusion period (D + I/R), and third diabetic group was subjected to 30 min of hepatic ischemia followed by a 45-min reperfusion period and treated with 20 mg/kg/day oral RSV before 30 min I/R injury (D + I/R + RSV). At the end of the experimental period, animals were decapitated, and blood samples were collected to determine tissue tumor necrosis factor-α (TNF-α) levels. Liver and lung tissue samples were obtained for the evaluation of biochemical parameters including malondialdehyde (MDA) and glutathione (GSH) levels and histopathological examinations. Compared to control, I/R injury resulted in decreases in GSH levels and increases in MDA levels. Tissue TNF-α levels were also increased in the D + I/R group compared to D group. Treatment with RSV prevented the alterations on biochemical parameters and histopathological changes induced by I/R. We demonstrate that in diabetic rats, hepatic I/R injury is associated with an augmented inflammatory response and oxidative stress, while RSV pre-treatment significantly decreased these responses. Larger clinical studies are desirable to determine the exact role(s) of RSV on hepatic I/R injury among diabetic subjects.