Prevalence and characteristics of Shiga-toxin producing Escherichia coli (STEC) isolated from beef slaughterhouse

• Published in: Journal of advanced veterinary and animal research Vol. 5; no. 3; pp. 218 - 225
• Main Authors: Parvej, Md, Mamun, Montasir, Hassan, Jayedul, Mahmud, Md, Rahman, Marzia, Rahman, Md, Nazir, K
• Format: Journal Article
• Language: English
• Published: Mymensingh Network for the Veterinarians of Bangladesh Bangladesh Agricultural Universityת Faculty of Veterinary Science 01.06.2018; Network for the Veterinarians of Bangladesh
• Subjects: Abattoirs; Animals; Antimicrobial agents; Bacteria; Beef; Biochemical tests; Cattle; Contamination; Correlation coefficient; Correlation coefficients; Diarrhea; Drug resistance; E coli; E. coli; Shiga-toxin; PCR; Stx1; Stx2; Epidemiology; Escherichia coli; Fecal coliforms; Feces; Food; Illnesses; Infectious diseases; Inflammatory bowel disease; Laboratories; Multidrug resistance; Pathogens; Polymerase chain reaction; Primers; Public health; Rectum; Stx1 gene; Stx2 gene; Bangladesh; Vietnam
• ISSN: 2311-7710; 2311-7710
• DOI: 10.5455/javar.2018.e271

Shiga-toxin producing Escherichia coli (STEC) is the most important foodborne bacterial pathogen worldwide and the bovine animals are assumed as a reservoir of this pathogen. The present study was conducted to assess the role of bovine animals as the source of STEC. To assess the role of bovine animals as the source of STEC, we examined 100 samples (50 rectal swab and 50 beef samples) collected from the local beef slaughterhouses by cultural, morphological, biochemical tests and polymerase chain reaction. Finally, the drug resistance pattern of isolated organisms has been examined. In the preliminary screening by polymerase chain reaction (PCR), E. coli was more prevalent in rectal swab (n=21/50) than beef samples (n=16/50). Among 39 isolated E. coli, 10 isolates were confirmed as STEC (Rectal swab=7, Beef=3) by PCR, where stx2 gene (n=7/10) was predominant than stx1 gene (n=3/10). Remaining 29 isolates did not react to stx primers in PCR. Presence of STEC in beef samples was significantly associated with the fecal contamination at P<0.1 (0.074818) in Pearson's correlation coefficient method. In addition, most of the isolated STEC strains were resistant to one or more commonly used antimicrobials in the country. The bovine animals and its products could be an important source of multidrug-resistant STEC in the country.