Dual-stimuli-responsive porous polymer enzyme reactor for tuning enzymolysis efficiency

• Published in: Mikrochimica acta (1966) Vol. 188; no. 12; p. 435
• Main Authors: Shen, Ji, Qiao, Juan, Zhang, Xinya, Qi, Li
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.12.2021; Springer; Springer Nature B.V
• Subjects: Amino acid oxidase; Amino acids; Analytical Chemistry; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Efficiency; Electrophoresis; Enzymes; Enzymes, Immobilized - metabolism; High temperature; Isopropylacrylamide; Light irradiation; Maleic anhydride; Membranes; Microengineering; Nanochemistry; Nanotechnology; Original Paper; Oxidases; Polymer industry; Polymers; Porosity; Stimuli; Stimuli Responsive Polymers - metabolism; Substrates; Ultraviolet radiation; Porous polymer membrane enzyme reactor; Tuning enzymolysis efficiency; D-amino acid oxidase; Capillary electrophoresis; Dual-stimuli-response
• ISSN: 0026-3672; 1436-5073
• DOI: 10.1007/s00604-021-05095-3

A strategy for preparing a dual-stimuli-responsive porous polymer membrane enzyme reactor (D-PPMER) is described, consisting of poly (styrene-maleic anhydride-N-isopropylacrylamide-acrylate-3′,3′-dimethyl-6-nitro-spiro[2H-1-benzopyran-2,2′-indoline]-1′-esterspiropyran ester) [P(S-M–N-SP)] and D-amino acid oxidase. Tunable control via “on/off” 365 nm UV light irradiation and temperature variation was used to change the membrane surface configuration and adjust the enzymolysis efficiency of the D-PPMER. A chiral capillary electrophoresis technique was developed for evaluation of the enzymatic efficiency of D-PPMER with a Zn(II)-dipeptide complex as the chiral selector and D,L-serine as the substrate. Interestingly, the enzymatic kinetic reaction rate of D-PPMER under UV irradiation at 36 °C (9.2 × 10 −2  mM·min −1 ) was 3.2-fold greater than that of the free enzyme (2.9 × 10 −2  mM·min −1 ). This was because upon UV irradiation at high temperature, the P(SP) and P(N) moieties altered from a “stretched” to a “curled” state to encapsulate the enzyme in smaller cavities. The confinement effect of the cavities further improved the enzymatic efficiency of the D-PPMER. This protocol highlights the outstanding potential of smart polymers, enables tunable control over the kinetic rates of stimuli-responsive enzyme reactors, and establishes a platform for adjusting enzymolysis efficiency using two different stimuli. Graphical abstract