Reversible immobilization of invertase on Cu-chelated polyvinylimidazole-grafted iron oxide nanoparticles
Polyvinylimidazole (PVI)-grafted iron oxide nanoparticles (PVIgMNP) were prepared by grafting of telomere of PVI on the iron oxide nanoparticles. Different metal ions (Cu 2+ , Zn 2+ , Cr 2+ , Ni 2+ ) ions were chelated on polyvinylimidazole-grafted iron oxide nanoparticles, and then the metal-chelat...
Saved in:
Published in | Bioprocess and biosystems engineering Vol. 36; no. 12; pp. 1807 - 1816 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.12.2013
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Polyvinylimidazole (PVI)-grafted iron oxide nanoparticles (PVIgMNP) were prepared by grafting of telomere of PVI on the iron oxide nanoparticles. Different metal ions (Cu
2+
, Zn
2+
, Cr
2+
, Ni
2+
) ions were chelated on polyvinylimidazole-grafted iron oxide nanoparticles, and then the metal-chelated magnetic particles were used in the adsorption of invertase. The maximum invertase immobilization capacity of the PVIgMNP–Cu
2+
beads was observed to be 142.856 mg/g (invertase/PVIgMNP) at pH 5.0. The values of the maximum reaction rate (
V
max
) and Michaelis–Menten constant (Km) were determined for the free and immobilized enzymes. The enzyme adsorption–desorption studies, pH effect on the adsorption efficiency, affinity of different metal ions, the kinetic parameters and storage stability of free and immobilized enzymes were evaluated. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1615-7591 1615-7605 |
DOI: | 10.1007/s00449-013-0955-x |