Reversible immobilization of invertase on Cu-chelated polyvinylimidazole-grafted iron oxide nanoparticles

• Published in: Bioprocess and biosystems engineering Vol. 36; no. 12; pp. 1807 - 1816
• Main Authors: Uzun, Kerem, Çevik, Emre, Şenel, Mehmet, Baykal, Abdülhadi
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.12.2013; Springer Nature B.V
• Subjects: Adsorption; beta-Fructofuranosidase - chemistry; Bioengineering; Biotechnology; Chelating agents; Chelating Agents - chemistry; Chemistry; Chemistry and Materials Science; Copper; Copper - chemistry; Environmental Engineering/Biotechnology; Enzymes, Immobilized - chemistry; Ferric Compounds - chemistry; Food Science; Hydrogen-Ion Concentration; Imidazoles - chemistry; Industrial and Production Engineering; Industrial Chemistry/Chemical Engineering; Iron alloys; Iron oxides; Metal ions; Metal Nanoparticles; Microscopy, Electron, Transmission; Nanoparticles; Original Paper; Polymers; Polyvinyls - chemistry; Temperature; Immobilization; Enzymes; Magnetic nanopartiecles; Sucrose; Metal chelation; Bioseparation
• ISSN: 1615-7591; 1615-7605
• DOI: 10.1007/s00449-013-0955-x

Polyvinylimidazole (PVI)-grafted iron oxide nanoparticles (PVIgMNP) were prepared by grafting of telomere of PVI on the iron oxide nanoparticles. Different metal ions (Cu 2+ , Zn 2+ , Cr 2+ , Ni 2+ ) ions were chelated on polyvinylimidazole-grafted iron oxide nanoparticles, and then the metal-chelated magnetic particles were used in the adsorption of invertase. The maximum invertase immobilization capacity of the PVIgMNP–Cu 2+ beads was observed to be 142.856 mg/g (invertase/PVIgMNP) at pH 5.0. The values of the maximum reaction rate ( V max ) and Michaelis–Menten constant (Km) were determined for the free and immobilized enzymes. The enzyme adsorption–desorption studies, pH effect on the adsorption efficiency, affinity of different metal ions, the kinetic parameters and storage stability of free and immobilized enzymes were evaluated.